[Histonet] fat cryostat sections: cutting pb, IHC?
Alan Bright
abright <@t> brightinstruments.com
Wed Apr 21 06:48:54 CDT 2004
Nancy,
The sample you are trying to section should not be a problem, it looks to me that your knife temperature and anti-roll plate are not cold enough, you omitted this temperature in your email perhaps you could inform me. Also you may need to have the specimen slightly warmer. I hope this assists you.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com
-----Original Message-----
From: Nancy.Walker <@t> sanofi-synthelabo.com [mailto:Nancy.Walker <@t> sanofi-synthelabo.com]
Sent: 20 April 2004 15:53
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fat cryostat sections: cutting pb, IHC?
Any pointers for cutting adipose tissue with a cryostat?
I have mouse epididyme adipose tissue that is unfixed and embedded in tissue tek. At - 30°C I can cut without it melting but most of the specimen stays on the knife edge leaving a nicely cut section of tissue tek, a big hole and a little fat tissue around the edges. And this at 45 µ!!! Any thinner and I get just the hole.
I have a crostat which I can adjust the block and chamber temp separately. Haven't fiddled with the knife angle yet...
WHAT TO DO???
any special tratments to get IHC reagents to penetrate this hydrophobic stuff?
thank you for you advice,
Nancy Walker
Molecular Pharmacology
Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE
nancy.walker <@t> sanofi-synthelabo.com
tel : (33)561004179 fax :(33)561004001
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