[Histonet] RE: Urea disclosure of endog biotin

[C.M. van der Loos]

Hi Carl,
In my hands on human kidney FFPE sections it's just the other way around: after citrate 6.0 retrieval there is hardly any endog. biotin (like with untreated sections) but after EDTA 9.0 there is lots of endog. biotin around again! 
Who ever said that IHC is a simple and straightforward technique???

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  Carl <carl.hobbs <@t> kcl.ac.uk> 
Date  Mon, 19 Apr 2004 21:06:04 +0100 
To  histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] re Urea disclosure of endog biotin 
Mmmmm...I have to "disclose" that citric acid M/Wing at pH6 does lead to endog biotin disclosure yet, when I do same with TRIS adjusted to pH10, I lose practically all endog biotin. (chick tissue being a perfect example)