[Histonet] VEGF

[Patsy Ruegg]

Luis,
I have not tried the mono vegf on mice but I have used it successfully on
rat tissue.  In my hands the poly vegf is so non-specific I could not ever
get it cleaned up enough to be worth anything.
Patsy

-----Original Message-----
From: Luis Chiriboga [mailto:Luis.Chiriboga <@t> med.nyu.edu]
Sent: Monday, April 12, 2004 9:39 AM
To: Patsy Ruegg; Mass Histology Service; Amos Brooks;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


Have any of you tried it on mouse?????? I have stuck with the polyclonal (on
mouse tissue) to avoid the species issue but it's so dirty anyway.  I'm
figuring I can at least try and manipulate the species issue (high dilution,
absorbed secondary, blocking, biotinylating primary etc...) and get better
results. Would you recommend?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Patsy
Ruegg
Sent: Friday, April 09, 2004 6:17 PM
To: Mass Histology Service; Amos Brooks;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


Yep that was my experience, this was the most drastic difference between a
monoclonal and polyclonal antibody I have ever seen.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Mass
Histology Service
Sent: Friday, April 09, 2004 2:58 PM
To: Patsy Ruegg; Amos Brooks; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


We just completed a large study on human prostate biopsies using the Santa
Cruz monoclonal VEGF (C1) following pronase pre-treatment and they all came
out  wonderful:  Very clean and the negative controls (no primary) were
completely negative.

Jim

____________________
James Staruk, HT(ASCP)
Mass Histology Service
www.masshistology.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Patsy
Ruegg
Sent: Friday, April 09, 2004 3:38 PM
To: Amos Brooks; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


Amos,
I totally agree with you about Santa Cruz's poly VEGF but the mono was much
better.  I have a customer who still wants to use the poly vegf on sheep
placenta and I think it is awful but you have to give the customer what they
want.  I did a comparison with the poly and mono for them but they still
opted for the poly because it matched their molecular studies.  Oh well.  To
me it is all over the place but they seem to think that is expected for
their samples, they think is is real and call it "an expression antibody" or
something like that.
Patsy

(snip)



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