[Histonet] BCL2 High pH retrieval

[Amos Brooks]

Hi,
         Try High pH antigen retrieval (pH 10.0). The steamer for 1/2 hour 
should be fine. I can't avouch for the detection kit you're stuck using 
with a Ventana stainer, but it should work. I would suggest a non biotin 
detection kit like Envision from DAKO or Mach3 from Biocare (I'm sure there 
are others). It seems high pH retrieval increases endogenous biotin.
Amos Brooks

At 09:20 PM 4/8/2004, you wrote:
>Message: 22
>Date: Fri, 9 Apr 2004 18:51:44 -0400
>From: "Derek & Lynda Leopold" <leopold <@t> mnsi.net>
>Subject: [Histonet] bcl-2 on Nexes
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <000801c41e85$3bc3fc40$3643fea9 <@t> leopold>
>Content-Type: text/plain;       charset="iso-8859-1"
>
>Hi All,
>We're having some trouble getting our tonsil control to come up for 
>bcl-2.  After searching the archives and asking around, we are going to 
>try citrate buffer (Declere) and a hot steamer for 1 hour.  We've got the 
>Nexes IHC.  Anyone else have any tips for us?
>Can anyone comment on the need/non-need for DAB?
>Thanks
>Lynda Leopold