[Histonet] Vibratome Considerations

[Danielle Zalinski]

  Hello All,
Our lab would like to do immunohistochem work in rat brain on vibratome 
sections instead of paraffin.  We are able to get sections about 
50-60um.  Will incubation with primary antibody overnight be sufficient? 
 Is shaking the sections an option?  I was hoping some other labs that 
are working in vibratome sections may have some beginners advice.
Thanks,
Danielle Zalinski

Neurosurgery Research
Henry Ford Health System
Detroit, MI


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