[Histonet] About H2O2 and CD Markers
Diane G. Miller
miller <@t> coho.net
Mon Apr 12 06:37:31 CDT 2004
Hi Jorge,
Sorry for the delay in getting this to you. Just two notes for your review.
Sodium Azide used in TBS buffer, plus H202 for FS only. This eliminated the air bubbles that can occur with Frozen Sections.
a.. Do not use methanol on frozen sections, it destroys the antigen sites and you will get a false negative.
Best Regards
Diane
----- Original Message -----
From: Jorge Ivan Zapata Valencia
To: histonet <@t> lists.utsouthwestern.edu
Sent: Friday, March 26, 2004 5:08 PM
Subject: [Histonet] About H2O2 and CD Markers
Hello everybody.
I have a simple question: do someone know the effect, if any, of the
different concentrations, and times, of H2O2 on the CD markers (Paraffin
and/or frozen sections)? I have read that some people use 0.01%, others 0.1%
and others 3% for different times, usually 10 or 30 minutes. What could
happen if I use a longer time?
Thank You very much.
JORGE IVAN ZAPATA
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