[Histonet] porcine insulin

[Jan Shivers]

Yes, a biotinylated anti-rabbit IgG does cross-react with guinea pig
antibodies.  I use it all the time when I stain for Insulin, using Dako's
guinea pig anti-Insulin product.

Jan Shivers
U of MN Vet Diag Lab

----- Original Message ----- 
From: "Kim Merriam" <kmerriam2003 <@t> yahoo.com>
To: "Stryker, S. (HLK)" <S.Stryker <@t> lumc.nl>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 06, 2004 1:59 PM
Subject: Re: [Histonet] porcine insulin


> I would use a biotinylated anti-guinea pig antibody.  I'm not sure if the
anti-rabbit antibody would bind the the GP primary antibody you are using.
I'm pretty sure that Vector sells an anti-GP secondary antibody (I think
they also sell a guinea pig ABC and alk phos kit, depending on the detection
system you are using).
>
> Good luck,
>
> Kim Merriam'
> Novartis
> Cambridge, MA
>
> "Stryker, S. (HLK)" <S.Stryker <@t> lumc.nl> wrote:
> Hello everybody,
>
> I'm hoping someone can give me any suggestions on how to detect insulin on
a
> (paraffin embedded formalin-fixed or frozen) porcine section using the
> following antibodies:
>
> - primary antibody: Guinea Pig anti-insulin (Dako)
> - secondary antibody: Swine anti-Rabbit (Dako)
>
> According to the DAKO catalogous the anti-Rabbit should work in this
combo.
> I have tried it over and over again, using different protocols, but
somehow
> I just can't get the job done...
>
> Thanks in advance!
>
> Samantha
> s.stryker <@t> lumc.nl
> Leiden University Medical Centre
>
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