[Histonet] porcine insulin
Kim Merriam
kmerriam2003 <@t> yahoo.com
Tue Apr 6 13:59:11 CDT 2004
I would use a biotinylated anti-guinea pig antibody. I'm not sure if the anti-rabbit antibody would bind the the GP primary antibody you are using. I'm pretty sure that Vector sells an anti-GP secondary antibody (I think they also sell a guinea pig ABC and alk phos kit, depending on the detection system you are using).
Good luck,
Kim Merriam'
Novartis
Cambridge, MA
"Stryker, S. (HLK)" <S.Stryker <@t> lumc.nl> wrote:
Hello everybody,
I'm hoping someone can give me any suggestions on how to detect insulin on a
(paraffin embedded formalin-fixed or frozen) porcine section using the
following antibodies:
- primary antibody: Guinea Pig anti-insulin (Dako)
- secondary antibody: Swine anti-Rabbit (Dako)
According to the DAKO catalogous the anti-Rabbit should work in this combo.
I have tried it over and over again, using different protocols, but somehow
I just can't get the job done...
Thanks in advance!
Samantha
s.stryker <@t> lumc.nl
Leiden University Medical Centre
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