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<TITLE>RE: [Histonet] optimal fixative for cytology smear?</TITLE>
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<P><FONT SIZE=2>if you are asking about fixative for immuno on cytology slides I suggest you methanol, slides have to be fixed immediately in cold methanol and they should also stay in methanol, they shouldn't be dried, you can store them up to 1 month for majority of markers with one exception - tDt, you have to stain it next day</FONT></P>
<P><FONT SIZE=2>hope this help, wish you happy holidays</FONT>
<BR><FONT SIZE=2>Irena Kirbis</FONT>
<BR><FONT SIZE=2>Cytopathology< department</FONT>
<BR><FONT SIZE=2>Ljubljana, Slovenia</FONT>
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<P><FONT SIZE=2>-----Original Message-----</FONT>
<BR><FONT SIZE=2>From: ulso [<A HREF="mailto:whatisdog@ksvcp.com">mailto:whatisdog@ksvcp.com</A>]</FONT>
<BR><FONT SIZE=2>Sent: Wednesday, December 24, 2003 6:47 AM</FONT>
<BR><FONT SIZE=2>To: histonet@lists.utsouthwestern.edu</FONT>
<BR><FONT SIZE=2>Subject: [Histonet] optimal fixative for cytology smear?</FONT>
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<P><FONT SIZE=2>Hi, histolanders!</FONT>
<BR><FONT SIZE=2>I am looking for adequate fixative appliable to cytology smear. In fact, I</FONT>
<BR><FONT SIZE=2>have tried acetone 100% 5 min so far, but repeatedly, I have found this</FONT>
<BR><FONT SIZE=2>fixative have poorly preserved the nuclear morphology, and somewhat dirty</FONT>
<BR><FONT SIZE=2>backgroud from celluar debris from fixation process.</FONT>
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<P><FONT SIZE=2>My topic is usually, CD antibodies such as CD3, 79a, 45RA, 1, 4, and 8 on</FONT>
<BR><FONT SIZE=2>canine lymph node or blood cells.</FONT>
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<P><FONT SIZE=2>Anyone out there recommend me any fixatives or tips on it? Literature based</FONT>
<BR><FONT SIZE=2>information would also be appreciated.</FONT>
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<P><FONT SIZE=2>Thank you and happy hollidays!</FONT>
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<P><FONT SIZE=2>Ul Soo Choi.</FONT>
<BR><FONT SIZE=2>DMV. graduate student</FONT>
<BR><FONT SIZE=2>Dept of clinical patholoy, CVM, Seoul Nat'l Univ.</FONT>
<BR><FONT SIZE=2>Seoul, Korea.</FONT>
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