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<TITLE>RE: [Histonet] RE: IHC on cells on coverslips</TITLE>
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<P><FONT SIZE=2>however, we got nice S-100 positive staining on many many Papanicolaou stained smears and cytospins. we use S-100 on Papanicolaou stained slides since 1990! </FONT></P>
<P><FONT SIZE=2>Irena Kirbis</FONT>
<BR><FONT SIZE=2>Cytopathology dept</FONT>
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<BR>
<P><FONT SIZE=2>-----Original Message-----</FONT>
<BR><FONT SIZE=2>From: Tony Henwood [<A HREF="mailto:AnthonyH@chw.edu.au">mailto:AnthonyH@chw.edu.au</A>]</FONT>
<BR><FONT SIZE=2>Sent: Monday, December 15, 2003 4:35 AM</FONT>
<BR><FONT SIZE=2>To: Histology Net List Server (E-Mail)</FONT>
<BR><FONT SIZE=2>Cc: 'Nader, Alexander'</FONT>
<BR><FONT SIZE=2>Subject: RE: [Histonet] RE: IHC on cells on coverslips</FONT>
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<P><FONT SIZE=2>Alexander,</FONT>
</P>
<P><FONT SIZE=2>Yes, granted S100 is alcohol soluble, but for melanoma confirmation, one can</FONT>
<BR><FONT SIZE=2>use HMB-45 or Melan-A.</FONT>
<BR><FONT SIZE=2>Remember, sometimes one may only have one or two cell smears available in</FONT>
<BR><FONT SIZE=2>order to confirm a diagnosis (eg no material in the cell block) so one must</FONT>
<BR><FONT SIZE=2>resort to one of the available smears. In this instance, collecting evidence</FONT>
<BR><FONT SIZE=2>and expertise is important. We need to appreciate the usefulness and</FONT>
<BR><FONT SIZE=2>shortcommings of the antibodies we use.</FONT>
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<P><FONT SIZE=2>It does seem that there are more antibodies that work on ethanol fixed</FONT>
<BR><FONT SIZE=2>smears than those that do not.</FONT>
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<P><FONT SIZE=2>Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)</FONT>
<BR><FONT SIZE=2>Laboratory Manager</FONT>
<BR><FONT SIZE=2>The Children's Hospital at Westmead,</FONT>
<BR><FONT SIZE=2>Locked Bag 4001, Westmead, 2145, AUSTRALIA.</FONT>
<BR><FONT SIZE=2>Tel: 612 9845 3306</FONT>
<BR><FONT SIZE=2>Fax: 612 9845 3318</FONT>
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<P><FONT SIZE=2><A HREF="http://www.histosearch.com/homepages/TonyHenwood/default.html" TARGET="_blank">http://www.histosearch.com/homepages/TonyHenwood/default.html</A></FONT>
<BR><FONT SIZE=2><A HREF="http://us.geocities.com/tonyhenwoodau/index.html" TARGET="_blank">http://us.geocities.com/tonyhenwoodau/index.html</A></FONT>
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<P><FONT SIZE=2>-----Original Message-----</FONT>
<BR><FONT SIZE=2>From: Nader, Alexander [<A HREF="mailto:alexander.nader@wgkk.sozvers.at">mailto:alexander.nader@wgkk.sozvers.at</A>]</FONT>
<BR><FONT SIZE=2>Sent: Friday, 12 December 2003 11:36 PM</FONT>
<BR><FONT SIZE=2>To: Histology Net List Server (E-Mail)</FONT>
<BR><FONT SIZE=2>Subject: [Histonet] RE: IHC on cells on coverslips</FONT>
</P>
<BR>
<P><FONT SIZE=2>> "Not really beneficial to many antigens"? I could list at least 50</FONT>
<BR><FONT SIZE=2>> antibodies that work on ethanol fixed smears. Most of these </FONT>
<BR><FONT SIZE=2>> are in routine</FONT>
<BR><FONT SIZE=2>> use.</FONT>
<BR><FONT SIZE=2>> eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA,</FONT>
<BR><FONT SIZE=2>> Skeletal muscle myosin, Actin,etc etc.</FONT>
<BR><FONT SIZE=2>> </FONT>
</P>
<P><FONT SIZE=2>S100-Antigen, GCFBP and some other ab's "leach out" with ethanol fixation,</FONT>
<BR><FONT SIZE=2>even fixation of trephines with Schaffer's solution (ethanol/formalin</FONT>
<BR><FONT SIZE=2>mixture) shows this unwanted and rather nasty effect.</FONT>
</P>
<P><FONT SIZE=2>Alexander Nader MD</FONT>
<BR><FONT SIZE=2>Vienna, Austria</FONT>
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