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<p class=MsoNormal><span class=EmailStyle18><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Arial'>Tom,<o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailStyle18><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Arial'>It sounds
like you don’t have your components insinc.<span style="mso-spacerun:
yes"> </span>You must have enough activator (usually benzoyl peroxide) to
react with the initiator (usually nn-dimethyl aniline and polyethylene
glycol).<span style="mso-spacerun: yes"> </span>Polymerization requires
that the proper chemistry, volume and temperature combination be used.<span
style="mso-spacerun: yes"> </span>Perhaps it is too cold for your
chemical polymerization reaction to begin with the volume of mix you are using.<o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailStyle18><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Arial'>Patsy <span
style="mso-spacerun: yes"> </span><o:p></o:p></span></font></span></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;color:black'>-----Original
Message-----<br>
<b><span style='font-weight:bold'>From:</span></b>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<b><span style='font-weight:
bold'>On Behalf Of </span></b>Tom Schaer<br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, December 10, 2003
7:32 AM<br>
<b><span style='font-weight:bold'>To:</span></b>
histonet@lists.utsouthwestern.edu<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] polymerization
problems</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><![if !supportEmptyParas]> <![endif]><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'>Dear
Histonetters:</span></font><font color=black><span style='color:black;
mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'> </span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'>I am
going to try again, realizing that not many have vast experience with embedding
large specimen with technovit 9100 yet. Maybe someone may be able to
share some pointers for a non-profit lab?</span></font><font color=black><span
style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'> </span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'>Essentially,
we fail to successfully polymerize med fem ovine condyles in approx 100ml
jars. Tried at -15C with no change in solution consistency for a week;
then brought it up to -2C still no change over three days. Jars were
"vacuumed" and set in refrigerator / freezer. I am at the of my
rope of troubleshooting.</span></font><font color=black><span style='color:
black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'> </span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'>Thanks
for any pointers.</span></font><font color=black><span style='color:black;
mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'> </span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'>Tom</span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'>------------------------------------------</span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><strong><b><font size=1
color=black face="Times New Roman"><span style='font-size:7.5pt;color:black'>Thomas
P. Schaer, VMD</span></font></b></strong><font color=black><span
style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=1 color=black
face="Times New Roman"><span style='font-size:7.5pt;color:black'>Assist. Prof.
BIOMED, Drexel University<br>
Comparative Orthopaedic Research & Tissue Engineering<br>
Department of Clinical Studies<br>
University of Pennsylvania New Bolton Center<br>
382 West Street Road<br>
Kennett Square, PA 19348<br>
tel. 610-444-5800 (x6261 office)<br>
tel. 610-444-5800 (x6131 lab)<br>
fax. 610-925-8100<br>
</span></font><font color=black><span style='color:black'><a
href="mailto:tpschaer@mail.vet.upenn.edu"><font size=1><span style='font-size:
7.5pt'>tpschaer@mail.vet.upenn.edu</span></font></a></span></font><font
color=black><span style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
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