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<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><!-- Converted from text/rtf format -->I
think that the appropriate thing to do for increased background, rather than
attempt all kinds of methods, is to elicit the step at which problems are occurring
by use of appropriate controls. This will save you both time and money
ultimately.</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><br>
You can run all these in a single experiment – just make sure that the slides
are cut from the same specimen, which is known to stain positive. </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'> </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>There are quite a few causes of increased
background, of course – but this will allow you to find out with the
minimum of fuss, and within one day. </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'> </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate alone</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate + ABC</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate + ABC + secondary
antibody</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate + ABC + primary
antibody + secondary antibody </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate + ABC + primary
antibody + secondary antibody + peroxidase quenching</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate + ABC + primary
antibody + secondary antibody + blocking reageants</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Slide with substrate + ABC + primary
antibody + secondary antibody + peroxidase quench + blocking reageants. (dissimilar
species serum)</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'> </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Min-Han Tan</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Van Andel Research Institute</span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'> </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'> </span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> Scholz, Stephen J.
[mailto:Stephen.J.Scholz@osfhealthcare.org] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Friday, December 05, 2003
12:32 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Histonet@Pathology.swmed.edu<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] pretreatment
for IHC</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p style='margin-left:.5in'><font size=2 face=Arial><span style='font-size:
10.0pt;font-family:Arial'>Hello Histonet,</span></font> </p>
<p style='margin-left:.5in'><font size=2 face=Arial><span style='font-size:
10.0pt;font-family:Arial'>I am having a problem with background staining on IHC
slides. This is only happening in cell block specimens. Is this
common on cell blocks? Should I do a pretreatment on these? What
pretreatments do others prefer? Should I peroxide quench? Before or
after? What about blockers? Do I ramble on and ask to many
questions?</span></font></p>
<p style='margin-left:.5in'><font size=2 face=Arial><span style='font-size:
10.0pt;font-family:Arial'>Thanks for all your help,</span></font> </p>
<p style='margin-left:.5in'><font size=2 face=Arial><span style='font-size:
10.0pt;font-family:Arial'>Stephen J. Scholz HT(ASCP)</span></font> <br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Histology
Coordinator</span></font> <br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>OSF
St. Anthony Medical Center</span></font> <br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Rockford
IL</span></font> </p>
<p style='margin-left:.5in'><font size=2 face=Arial><span style='font-size:
10.0pt;font-family:Arial'>Phone: 815-395-5410</span></font> <br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Fax:
815-395-5364</span></font> <br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>e-mail:
sjscholz@osfhealthcare.org</span></font> </p>
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