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<DIV><FONT face=Arial size=2>By better refractive index, I presume you mean
higher refractive index. If that is correct, the aqueous medium I used
when I wanted good clarity was PVP glycerol. I have no reference, nor do I
know who introduced it, but the recipe is:</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Polyvinylpyrrolidone 50 grams</FONT></DIV>
<DIV><FONT face=Arial size=2>Distilled water 50 ml</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Stir the two together, and put in a 56C incubator
until it is a clear syrup. Add 10 mL glycerol, mix very well. Leave
another hour or so for bubbles to rise, then cool to room temperature. In
a capped container it will last for years.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Almost dry sections, place the medium onto the
center of the section, not the edge of the slide. Apply coverslip.
Do not press. Make any adjustments to the coverslip immediately as it sets
in about five minutes. Its disadvantage is that it slowly dries back over
some months.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>I used this medium for muscle biopsy enzymes and it
worked very well.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Bryan Llewellyn</FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=dave_laidley@yahoo.ca href="mailto:dave_laidley@yahoo.ca">David
Laidley</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A
title=histonet@lists.utsouthwestern.edu
href="mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouthwestern.edu</A>
</DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Tuesday, December 02, 2003 9:20
AM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> [Histonet] aqueous mounting
medium nad refractive index</DIV>
<DIV><BR></DIV>
<DIV>Does anyone know the refractive index of Faramount (Dako) and
PVA-glycerol, both aqueous mounting mediums.</DIV>
<DIV> </DIV>
<DIV>Can anyone suggest an aqueous mounting medium that has a better
refractive index than the ones mentioned above (if available)</DIV>
<DIV> </DIV>
<DIV>David Laidley</DIV>
<DIV>Memorial University of Newfoundland</DIV>
<DIV>Division of Basic Medical Sciences</DIV>
<DIV>St. John's, Newfoundland</DIV>
<DIV> </DIV>
<DIV><A href="mailto:dave_laidley@yahoo.ca">dave_laidley@yahoo.ca</A></DIV>
<DIV> </DIV>
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