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<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=630402823-20112003>I have
used both types and agree with the comments made by Charles. Some further points:</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=630402823-20112003>1. Closed systems process large
blocks (eg brain) and fatty tissues better than open systems due to the stronger
pressure and vacuum that can be applied.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=630402823-20112003>2. To obtain equivalent processing
quality from an open system requires at least double the processing
time.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=630402823-20112003>3. Because open systems are more
gentle on tissues, small endoscopics and core biopsies process better than with
closed systems.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=630402823-20112003>4. The choice? For large throughput,
general path labs definitely consider at least one closed system processor and
for Children's Hospital labs (smaller biopsies), open system processors are definitely wothwhile.</SPAN></FONT></DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=630402823-20112003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial color=#0000ff size=2><SPAN class=630402823-20112003>
<P><FONT face=Arial size=2>Tony Henwood JP, B</FONT><FONT face=Arial size=2>A</FONT><FONT face=Arial size=2>ppSc, GradDipSysAnalys, CT(ASC)</FONT>
<BR><FONT face=Arial size=2>Laboratory Manager</FONT> <BR><FONT face=Arial
size=2>The Children's Hospital at Westmead,</FONT> <BR><FONT face=Arial
size=2>Locked Bag 4001, Westmead, 2145, AUSTRALIA.</FONT> <BR><FONT face=Arial
size=2>Tel:</FONT> <FONT face=Arial size=2>61</FONT><FONT face=Arial size=2>2
9845 3306</FONT> <BR><FONT face=Arial size=2>Fax:</FONT> <FONT face=Arial
size=2>61</FONT><FONT face=Arial size=2>2 9845 3318</FONT> </P>
<P><FONT face=Arial size=2><A target=_blank
href="http://www.histosearch.com/homepages/TonyHenwood/default.html">http://www.histosearch.com/homepages/TonyHenwood/default.html</A></FONT>
<BR><FONT face=Arial size=2><A target=_blank
href="http://us.geocities.com/tonyhenwoodau/index.html">http://us.geocities.com/tonyhenwoodau/index.html</A></FONT>
</P></SPAN></FONT></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Charles W. Scouten, Ph.D.
[mailto:cwscouten@myneurolab.com]<BR><B>Sent:</B> Friday, 21 November 2003
9:56 AM<BR><B>To:</B> JCarpenter764@aol.com;
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> RE: [Histonet] different
types of processors<BR><BR></FONT></DIV>
<DIV class=Section1>
<P class=MsoNormal><FONT face="Times New Roman" color=navy size=3><SPAN
style="FONT-SIZE: 12pt; COLOR: navy"></SPAN></FONT> </P>
<P class=MsoNormal><SPAN class=product><FONT face=Verdana size=3><SPAN
style="FONT-SIZE: 12pt; FONT-FAMILY: Verdana">There are many real advantages
to a dip and move system (OPEN) when compared to a sealed flush (CLOSED)
system. The following points highlight the operational differences 1) Time -
the minimum cycle time for a flush system is 2 hours. This is due to the heating phase for the paraffin and the additional rinse cycles. For quick
procedures such as </SPAN></FONT></SPAN><SPAN class=product><FONT
face=Verdana><SPAN style="FONT-FAMILY: Verdana">bio</SPAN></FONT></SPAN><SPAN
class=product><FONT face=Verdana><SPAN style="FONT-FAMILY: Verdana">psies, 2
hours is an inconvenient cycle time when it can be done in 30 minutes with a
TPC system. A procedure that would take only 8 hours with the TPC will take 12
hours in a sealed system. 2) Artifacts – In order to introduce the paraffin in
a sealed system, the chamber must be heated. This heating process can cause
dead spots, shadows, or artifacts in your tissue. The TPC system does not create such artifacts. 3) Maintenance – The TPC system does not rely on pumps
to complete the protocols. The sealed system requires pumps which can fail.
These can be costly and inconvenient to replace. 4) Contamination – Although
the sealed systems are thought to be contamination free, they do require cleaning after every protocol. Proteins are routinely washed out of sections
during processing. These proteins will settle in the bottom of flush storage
tanks and will be present for future protocols. In order to remove all
contaminates, the system must be cleaned. 5) Cost – The TPC system uses a much
lower volume of reagents and chemicals. These add up over the coarse of a year. Have you actually calculated your annual costs and looked at the potential savings? 6) Tissue Shrinking – The TPC system does not expose the
tissue to a heating cycle and therefore does not cause the tissue to
shrink.</SPAN></FONT></SPAN></P>
<P class=MsoNormal><SPAN class=product><FONT face=Verdana size=3><SPAN
style="FONT-SIZE: 12pt; FONT-FAMILY: Verdana"></SPAN></FONT></SPAN> </P>
<P class=MsoNormal><SPAN class=product><FONT face=Verdana size=3><SPAN
style="FONT-SIZE: 12pt; FONT-FAMILY: Verdana">I am also attaching a detailed
data sheet here. If you have any questions or would like to discuss this further please feel free to contact me anytime. </SPAN></FONT></SPAN></P>
<P class=MsoNormal><FONT face="Times New Roman" color=navy size=3><SPAN
style="FONT-SIZE: 12pt; COLOR: navy"></SPAN></FONT> </P>
<DIV>
<P><FONT face="Comic Sans MS" color=navy size=3><SPAN
style="FONT-SIZE: 12pt; COLOR: navy; FONT-FAMILY: 'Comic Sans MS'">Charles
W. Scouten, Ph.D.</SPAN></FONT><FONT color=navy><SPAN
style="COLOR: navy"> <BR></SPAN></FONT><B><FONT color=#339966><SPAN style="FONT-WEIGHT: bold; COLOR: #339966">myNeuroLab.com</SPAN></FONT></B><FONT
color=navy><SPAN style="COLOR: navy"> <BR></SPAN></FONT><FONT
face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">5918
Evergreen Blvd.</SPAN></FONT><FONT color=navy><SPAN style="COLOR: navy">
<BR></SPAN></FONT><FONT face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">St.
Louis</SPAN></FONT><FONT face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">,
</SPAN></FONT><FONT face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">MO</SPAN></FONT><FONT
face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'"> </SPAN></FONT><FONT face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">63134</SPAN></FONT><FONT
color=navy><SPAN style="COLOR: navy"> <BR></SPAN></FONT><FONT
face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">Ph: 314
522 0300 </SPAN></FONT><FONT color=navy><SPAN
style="COLOR: navy"><BR></SPAN></FONT><FONT face="Bookman Old Style" color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Bookman Old Style'">FAX
314 522 0377</SPAN></FONT><FONT color=navy><SPAN style="COLOR: navy"> <BR><A
href="mailto:cwscouten@myneruolab.com">cwscouten@myneurolab.com</A>
<BR>www.myneurolab.com </SPAN></FONT></P></DIV>
<P class=MsoNormal><FONT face="Times New Roman" color=navy size=3><SPAN
style="FONT-SIZE: 12pt; COLOR: navy"></SPAN></FONT> </P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma">-----Original
Message-----<BR><B><SPAN style="FONT-WEIGHT: bold">From:</SPAN></B>
JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] <BR><B><SPAN
style="FONT-WEIGHT: bold">Sent:</SPAN></B> </SPAN></FONT><FONT face=Tahoma
size=2><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma">Thursday, November
20, 2003</SPAN></FONT><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma"> </SPAN></FONT><FONT face=Tahoma
size=2><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma">3:10
PM</SPAN></FONT><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma"><BR><B><SPAN
style="FONT-WEIGHT: bold">To:</SPAN></B>
histonet@lists.utsouthwestern.edu<BR><B><SPAN
style="FONT-WEIGHT: bold">Subject:</SPAN></B> [Histonet] different types of
processors</SPAN></FONT></P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><FONT face="Times New Roman"
size=3><SPAN style="FONT-SIZE: 12pt"></SPAN></FONT> </P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">Would someone please explain the
difference between an open processor and a closed
processor....</SPAN></FONT></P></DIV></BLOCKQUOTE><CODE><FONT SIZE=3><BR>
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