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<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'>I have responded separately with an attached
7 page discussion of perfusion, and a link to our Perfusion One
product. Don’t use flow rate, pressure is what the cardiovascular
system controls, use 5% sucrose prewash, no saline, and switch to fixative
when the animal stops. Haparin is not needed, all blood can be cleared without
it with sufficient pressure.</span></font></p>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'> </span></font></p>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'>If any body else would like to see these
materials, I will send them.</span></font></p>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'> </span></font></p>
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<p><font size=3 color=navy face="Comic Sans MS"><span style='font-size:12.0pt;
font-family:"Comic Sans MS";color:navy'>Charles W. Scouten, Ph.D.</span></font><font
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</span></font><b><font color="#339966"><span style='color:#339966;font-weight:
bold'>myNeuroLab.com</span></font></b><font color=navy><span style='color:navy'>
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</span></font><font size=2 color=navy face="Bookman Old Style"><span
style='font-size:10.0pt;font-family:"Bookman Old Style";color:navy'>Ph: 314 522
0300 </span></font><font color=navy><span style='color:navy'><br>
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<a href="mailto:cwscouten@myneruolab.com">cwscouten@myneurolab.com</a> <br>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> Davis, Gareth
[mailto:gareth.davis@Vanderbilt.Edu] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Saturday, November 15, 2003
3:29 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Histonet<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] Perfusion pump
time</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Hello Histonetters,</span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Recently (within the last couple months) I saw someone
post perfusion times for pumps based on the animal being perfused. I
tried to find the posting on the Histosearch, but unsuccessful. We are
perfusing rats for the olfactory tissue, but would like ideals on the what
others have done. Any input would be appreciated, including if heparin is
needed in saline, the correct perfusion pump time, and if
recirculation is necessary.</span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>thanks,</span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Gareth</span></font></p>
</div>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 color=purple
face=System><span style='font-size:10.0pt;font-family:System;color:purple'>Ms.
Gareth B. Davis</span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><strong><b><font size=2
color=purple face=System><span style='font-size:10.0pt;font-family:System;
color:purple'>Research Assistant II</span></font></b></strong></p>
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