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<TITLE> Decreased antigenicity with alcohol-formalin fixation</TITLE>
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<P><FONT SIZE=2 FACE="Arial">Greetings, Histonetters.</FONT>
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<P><FONT SIZE=2 FACE="Arial">One of my pathologists has expressed concern that antigenicity for certain antibodies, specifically ER and PR, in breast tissue that is processed using alcohol-formalin will be decreased. Can anyone cite a reference in the literature that confirms or addresses this concern? Has anyone had this experience, or does anyone know of any reason that using alcohol-formalin (specifically Pen-Fix) would adversely affect IHC staining?</FONT></P>
<P><FONT SIZE=2 FACE="Arial">Thanks to all of you,</FONT>
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<P><I><FONT SIZE=2 FACE="Bookman Old Style">Diana Goodwin</FONT></I>
<BR><I><FONT SIZE=2 FACE="Bookman Old Style">Supervisor, Anatomic Pathology</FONT></I>
<BR><I><FONT SIZE=2 FACE="Bookman Old Style">Preston 6</FONT></I>
<BR><I><FONT SIZE=2 FACE="Bookman Old Style">215-829-6532</FONT></I>
<BR><I><FONT SIZE=2 FACE="Bookman Old Style">e-mail: goodwind@pahosp.com</FONT></I>
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