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<P>Jason,</P></DIV>
<P>I found that dulling the blade with a Kimwipe knocks the edge off the blade down enough to get ribbons right away. Try not to soak the array block in water. If so, only for a few minutes - use straight ice. Sometimes the block will cut better once it gets almost to room temp. Also, if you are noticing any lines in the paraffin move to a new part of the blade or change to a new blade.</P></DIV>
<P>Thom</P>
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<P> </P>
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<P>For more information on Tissue Microarray instruction visit: <A href="http://www.arrayworkshop.com/">www.arrayworkshop.com</A></P>
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<P> </P>
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<DIV></DIV>>From: "jason madore" <KOSMICDOG@HOTMAIL.COM>
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<DIV></DIV>>To: histonet@lists.utsouthwestern.edu
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<DIV></DIV>>Subject: [Histonet] sectioning tissue mircroarrays
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<DIV></DIV>>Date: Fri, 07 Nov 2003 06:54:18 -0800
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<DIV></DIV>>Salut everyone,
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<DIV></DIV>>I am hoping that someone can give me pointers for sectioning of
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<DIV></DIV>>tissue microarrays. I am using leica RM2125 and the first array i
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<DIV></DIV>>need to section is a 0.06mm 400core ovarian serous tumour array. I
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<DIV></DIV>>have a problems with the cores rolling up as the section is cut.
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<DIV></DIV>>About half of the roled cores flatten out in the water bath but
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<DIV></DIV>>still losing half the array in this way is not good. Is the knife or
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<DIV></DIV>>some step prior to sectioning. The block was incubated at 37ds for
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<DIV></DIV>>half an hour. Any pointers on dealing with these problems or any
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<DIV></DIV>>other problems I might encounter would be greatly appreciated.
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<DIV></DIV>>Thanks in advance. I am of course checking the histonet archives as
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<DIV></DIV>>well.
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<DIV></DIV>>Ciao, J.
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