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<DIV><SPAN class=530580814-31102003><FONT face=Arial color=#0000ff size=4>Tap
water varies a little from one water system to another. It is usually a
very dilute solution of the carbonates and chlorides of calcium, sodium, and
copper. Its pH is usually a little over 7, so it acts as a very weak
base. (Mine is 7.4) It must be used when the protocol calls
for it. </FONT></SPAN></DIV>
<DIV><SPAN class=530580814-31102003><FONT face=Arial color=#0000ff
size=4>Distilled and deionized water contain so few ions that neither one will
act as a weak base. For most purposes, distilled and deionized water are
interchangeable. It is easier to contaminate a deionizing system, but one
can contaminate a distilled water system. Small, lab-sized systems are
less likely to become contaminated with bacteria or minerals than large
building-sized systems are. </FONT></SPAN></DIV>
<DIV><SPAN class=530580814-31102003><FONT face=Arial color=#0000ff size=4>If you
need really pure water, boil 600 ml of deionized water and add 6 mg of potassium
permanganate to it and distill the water. </FONT></SPAN><SPAN
class=530580814-31102003><FONT face=Arial color=#0000ff size=4>Discard the first
100 ml of distillate. The next 300 ml will be very pure water, but I
can think of no biological use for it. (Such very pure water is sometimes
used for physics experiments.)</FONT></SPAN></DIV>
<DIV><SPAN class=530580814-31102003><FONT face=Arial color=#0000ff size=4>Both
distilled and deionized water pick up carbon dioxide on standing and become weak
solutions of carbonic acid. If you work alone, its pH will probably be
6.9; if there are several people in the lab, the pH may fall to
6.8.</FONT></SPAN></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B> Morken, Tim -
Labvision [mailto:tpmorken@labvision.com] <BR><B>Sent:</B> Tuesday, October
28, 2003 12:53 PM<BR><B>To:</B> 'JCarpenter764@aol.com';
histonet@lists.utsouthwestern.edu<BR><B>Subject:</B> RE: [Histonet] Dionized
vs distilled water<BR><BR></FONT></DIV>
<DIV class=Section1>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial">Distilled water is
classically produced by heating water to evaporation and subsequent condensing
on a cold surface. In the process most impurities are either evaporated off
ahead of the water (in the case of most organics), or left behind (in the case
of minerals). The water is also effectively <SPAN
class=SpellE>deionized</SPAN> because the salts are left behind. It is fairly
pure water. To get very pure water it needs to be re-distilled several
times.<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><SPAN class=SpellE><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial">Deionized</SPAN></FONT></SPAN><FONT
face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial"> water is classically
passed through a salt bed or ionized resin bed that captures the mineral ions
(<SPAN class=SpellE>ie</SPAN>, a "water softener"). The water is not
necessarily pure, however, especially in regards to organic chemicals. Reverse
osmosis is also used now days to <SPAN class=SpellE>deionize</SPAN>
water.<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial">High quality water
systems these days are some combination of filters, distillation, <SPAN
class=SpellE>deionizing</SPAN> resins and reverse osmosis.
<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoAutoSig><FONT face="Courier New" color=navy size=3><SPAN
style="FONT-SIZE: 12pt; COLOR: navy; FONT-FAMILY: 'Courier New'; mso-no-proof: yes">Tim
Morken<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial color=navy size=2><SPAN
style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: Arial"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma">-----Original
Message-----<BR><B><SPAN style="FONT-WEIGHT: bold">From:</SPAN></B>
JCarpenter764@aol.com [mailto:JCarpenter764@aol.com<SPAN class=GramE>]
<BR><B><SPAN style="FONT-WEIGHT: bold">Sent</SPAN></B></SPAN><B><SPAN
style="FONT-WEIGHT: bold">:</SPAN></B> </SPAN></FONT><st1:date
style="BACKGROUND-POSITION: left bottom; BACKGROUND-IMAGE: url(res://ietag.dll/#34/#1001); BACKGROUND-REPEAT: repeat-x"
Year="2003" Day="28" Month="10"><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma">Tuesday, October 28,
2003</SPAN></FONT></st1:date><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma"> </SPAN></FONT><st1:time
Minute="45" Hour="7"><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma">7:45
AM</SPAN></FONT></st1:time><FONT face=Tahoma size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Tahoma"><BR><B><SPAN
style="FONT-WEIGHT: bold">To:</SPAN></B>
histonet@lists.utsouthwestern.edu<BR><B><SPAN
style="FONT-WEIGHT: bold">Subject:</SPAN></B> [Histonet] (no
subject)</SPAN></FONT></P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><FONT face="Times New Roman"
size=3><SPAN style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><FONT face=Arial size=2><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">while studying for my exam on the
different fixatives and there ingredients....i have noticed that some call for
distilled water and some use the term deionized water. Is there a
difference?<o:p></o:p></SPAN></FONT></P></DIV></BLOCKQUOTE> <br> <br> <br>The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.<br>Barry University - Miami Shores, FL (http://www.barry.edu) </BODY></HTML>