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<DIV style="FONT: 10pt arial">----- Original Message -----
<DIV style="BACKGROUND: #e4e4e4; font-color: black"><B>From:</B> <A
title=bryand@netbistro.com href="mailto:bryand@netbistro.com">Bryan
Llewellyn</A> </DIV>
<DIV><B>To:</B> <A title=ian.montgomery@bio.gla.ac.uk
href="mailto:ian.montgomery@bio.gla.ac.uk">Ian Montgomery</A> </DIV>
<DIV><B>Sent:</B> Wednesday, October 29, 2003 9:48 AM</DIV>
<DIV><B>Subject:</B> Re: [Histonet] Sirius Red Technique.</DIV></DIV>
<DIV><BR></DIV>
<DIV><FONT face=Arial size=2>It very slowly deteriorates over time, but it can
be compensated for by extending staining time. I have used solutions a
year old with 2 hours staining, but that was stretching it a bit. Six
months would be OK. So one hour fresh, 2 hours at six months.
Incidentally, it is better if no more than 2 mL salt solution is added.
The absolute minimum salt is the critical step.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Bryan Llewellyn</FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=ian.montgomery@bio.gla.ac.uk
href="mailto:ian.montgomery@bio.gla.ac.uk">Ian Montgomery</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A
title=histonet@lists.utsouthwestern.edu
href="mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouthwestern.edu</A>
</DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Wednesday, October 29, 2003 2:46
AM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> [Histonet] Sirius Red
Technique.</DIV>
<DIV><BR></DIV><X-TAB> </X-TAB>Quick
question and the man himself may be able to answer. Sirius Red Technique
(Llewellyn, 1970), what is the shelf life of the prepared
stain?<BR>Ian.<BR><X-SIGSEP>
<P></X-SIGSEP><FONT color=#0000ff size=3>Dr. Ian
Montgomery,<BR>Histotechnology,<BR>Graham Kerr Building,<BR>Institute of
Biomedical & Life Sciences,<BR>University of Glasgow,<BR>Glasgow,<BR>G12
8QQ.<BR>Tel: 0141 339 8855 <BR>Office: 4652<BR>Lab: 6644.<BR>Pager: 07625
702883<BR>e-mail: ian.montgomery@bio.gla.ac.uk</FONT>
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