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<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Folks<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>ABOUT COVER GLASS AND BUBBLES---<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Lee Luna---some of you may not have known Lee---he was editor
of the government histological manual---he came to visit us several times at </span></font><st1:place><st1:PlaceName><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Good</span></font></st1:PlaceName><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> </span></font><st1:PlaceName><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Samaritan</span></font></st1:PlaceName><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> </span></font><st1:PlaceType><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Hospital</span></font></st1:PlaceType></st1:place><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>.<span
style='mso-spacerun:yes'> </span>He loved an ice cream bar we could get
around here---called THAT’S <span class=GramE>IT,</span> or something
like that. He and I got off our diets a couple a time per visit with that. <span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>One visit, he showed us a way to cover slip slides that was
easier, faster and practically bubble proof.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>FIRST OF ALL--- WIPING THE SLIDES WITH A TOWEL IS <span
class=GramE>A <span style='mso-spacerun:yes'> </span>SILLY</span>
TIC.<span style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span>THERE IS MORE LINT AND STUFF PUT ONTO THE
SLIDES FROM A TOWEL THAN WOULD BE THERE FROM THE FINAL CLEARING AGENT. <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>You take two or three slides out of the clearing agent--- it
was <span class=SpellE>xylene</span> with us---one at a time-----<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>You lay them on two or three clean paper towels----you can
replace the paper towels any time they get too wet with <span class=SpellE>xylene</span>,
but you can do a lot of slides without doing so.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>This was done under the hood of course.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>You simply lay the slides out on the paper towels.<span
style='mso-spacerun:yes'> </span>AH <span class=SpellE>AH</span> <span
class=SpellE>AH</span>---PUT THAT HAND TOWEL AWAY!!!---<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>You put a streak of the cover glass mounting medium you use
on the upper or lower edge of the slides----which ever is more comfortable to
you. You will learn quickly how much mounting medium to put on the slides. You
then lay the cover glass one by one on the slides---if you put the mounting
medium on the upper edge of one slide---you lay the upper edge of the cover
glass in the mounting medium on the upper edge of that slide and lower it, leading
the mounting medium downward before it with no bubbles. If you do make bubbles,
you can draw them down and off the lower edge of the slide. You then do the
same for slides two and three. The ventilation in our lab was strong---I could
never do more than three slides at a time this way----the mounting medium would
be evaporating.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>This was quick and neat---and you could easily control
bubbles. <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>There are those who will insist on continuing to hold the
slides up in the air in front of their faces to cover them----folks, if you do---you
might as well cover slip while standing on your heads.<span
style='mso-spacerun:yes'> </span>Just breathe slowly, smile, and try the
above.<span style='mso-spacerun:yes'> </span>It’s hygienic and low
cal. <span style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>If you put the mounting medium on the lower edge of the
slide, you simply <span class=SpellE>appliy</span> the cover glass to the lower
edge and lower it up to the top of the slide leading any bubbles off the edge
of the slide. And, with either upper or lower edge application, it was easy to
learn how to put the cover slip on without making bubbles. But if you did, you
could scoot the bubbles off with ease when lowering the cover glass.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>There was a variation on this that some techs liked----you
lay the COVER GLASSES out on the paper towels, one two or three----put the
mounting medium on the upper or lower edges of each cover glass---than touch
the lower edge of the slide itself over the cover glass in the mounting medium---the
mounting medium will fill up the space naturally between the cover and the
slide with few bubbles resulting----if you do make bubbles, you simply lead
them off when you finish lowering the slide onto the cover glass---either from
the bottom up or from the top down.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>With all of the above, you finish with touching the bottom
of the slides to the paper towels to drain any excess mounting medium off the
slides.<span style='mso-spacerun:yes'> </span>NO CLOTH TOWELS HERE. There
are those who will prefer to touch both top and bottom of the slides to the
paper towels.<span style='mso-spacerun:yes'> </span>This is ok, but may not
be necessary. <span style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>You can do your covering quickly---neat, clean and bubble-free.<span
style='mso-spacerun:yes'> </span>I know from the <span class=SpellE>histonet</span>,
many of you have auto <span class=SpellE>stainers</span>, and some of you have
auto cover glassing equipment.<span style='mso-spacerun:yes'> </span>I
never did.<span style='mso-spacerun:yes'> </span>The equipment was ME. If
you can get bubble free cover slipping from those mechanical slaves, good. But
I can say that bubbles were not unusual with slides covered by motorized cover
slipping.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Like John Henry, I was ready to beat the machine with quantity
and quality but we never got one.<span style='mso-spacerun:yes'> </span>Shucks!
There might have been a song written about it---- <span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Georgecole@ev1.net <span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><span
style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
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