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<DIV><SPAN class=296030314-15102003><FONT face=Georgia>You might want to call
the author or contact person on the paper. Protocols are usually the first
on the cutting floor. Most people are delighted to have someone pursue their
research!</FONT></SPAN></DIV>
<DIV><SPAN class=296030314-15102003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=296030314-15102003><FONT face=Georgia>Good luck-
c</FONT></SPAN></DIV>
<DIV> </DIV>
<P><FONT face="BernhardFashion BT" color=#000080>Cynthia Favara</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>NIAID/NIH/RML/LPVD</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>903 South 4th Street</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>Hamilton, MT 59840</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>406-363-9317</FONT> </P>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Bernard Ian R SSgt 59
CRES/MSROP [mailto:Ian.Bernard@LACKLAND.AF.MIL]<BR><B>Sent:</B> Tuesday,
October 14, 2003 8:02 AM<BR><B>To:</B> Histonet (E-mail)<BR><B>Subject:</B>
[Histonet] Help; looking for a stain receipe for TTC<BR><B>Importance:</B>
High<BR><BR></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003>Hello folk or
fellow histonetters(in particular in research), </SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003>I'm in the
process of replicating the histochemical staining method in a protocol
article titled " LV-Powered Coronary Sinus Retroperfusion Reduces infarct
Size in Acutely Ischemic Pigs."</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003>The made reagents
for staining the heart tissue calls for a pre staining is a Phthalol-blue,
which we will be substituting with Evans Blue. <STRONG>I need a
concentration and receipe to make this up?</STRONG> <STRONG> The
article does not provide this. </STRONG> The article states that the
" ascending aorta was occluded and phthalo-blue(Evans Blue) was injected
into the left ventricle.</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003><STRONG>Finally
the article states</STRONG>: " The left Ventricle was sliced, parallel to the
atriopventricular groove, into several 5-10 mm slices and incubated for 30
mins at 37 deg C in TTC or Triphenyltetrazolium Chloride. <STRONG>There
are 2 concentrations by Sigma, which one is to be used here, a 95% or 99.5
%? Need receipe to make this solution?</STRONG></SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003><STRONG></STRONG></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003><STRONG>What is
Trisma HCL and Trisma Base? I need catalog #'s and
source?</STRONG></SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003><STRONG>The
article goes on to state for results</STRONG>:That "the Mycardial tissue
containing dehydrogenase enzymes is stained brick red by TTC, but areas of
necrotic tissue depleted of these enzymes are not stained and appear as white
or pale yellow."</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN
class=253534313-14102003>Thanks</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=253534313-14102003>SSgt
Bernie</SPAN></FONT></DIV></BLOCKQUOTE></BODY></HTML>