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<div class=Section1>
<ol style='mso-margin-top-alt:0in' start=1 type=1>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Even
when a substantial cubic cm piece of muscle was sent, tiny 1 and 2 mm bits
of tissue were sectioned. <o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Gum <span
class=SpellE>Tragacanth</span> was used to position the <span class=GramE>tissue<span
style='mso-spacerun:yes'> </span><span class=SpellE>Tragacanth</span></span>
helps get good sections., but it is a bit messy and it is itself a form of
sugar which tends to confound testing for abnormal glycogen.<o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>To hold
the tissue for freezing odd heavy metal plugs were used with gum <span
class=SpellE>tragacanth</span> and pip of muscle on top of it to insert
into the <span class=SpellE>Isopentane</span> and liquid nitrogen.<span
style='mso-spacerun:yes'> </span>The mass of metal commands the edge
of the cold effect, which tends to add to the threat of ice crystals.<o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Gum <span
class=SpellE>tragacanth</span> surrounding bits of tissue are placed on
cork to freeze.<span style='mso-spacerun:yes'> </span></span></font><st1:City><st1:place><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Cork</span></font></st1:place></st1:City><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> is a
very good insulator and adds a bit to the chance of ice crystals on the
underside of the tissue.<o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><span
class=SpellE><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Isopentane</span></font></span><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'> is cooled in <span
class=GramE>the<span style='mso-spacerun:yes'> </span>liquid</span>
nitrogen until it gets a little thick.<o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>The
tissues are sectioned in their native shape which will almost always be
odd, with planes and peeks of tissue. Sections are hardly at their best as
they almost always twist and curl onto the knife due to the erratic angles
presented to the knife. You can see the twisting wayward sections coming
onto the knife in some of the muscle text books.<o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Sections---for
some strange reason, perhaps due to the suggestion of a tricky leprechaun,
who is still laughing at us for taking him<span
style='mso-spacerun:yes'> </span>up on it----are picked up on cover
slips.<o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Amounts
of reagents are kept to the minimum----barely covering the sections on the
slide.<span style='mso-spacerun:yes'> </span><o:p></o:p></span></font></li>
<li class=MsoNormal style='mso-list:l1 level1 lfo3;tab-stops:list .5in'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><span
style='mso-spacerun:yes'> </span>The usual buffer for ATP-<span
class=SpellE>ase</span> mixtures continues to be the sodium pentobarbital
buffer in the literature.<span style='mso-spacerun:yes'> </span>This
takes a controlled substance permit to procure and it’s not worth
the trouble.<span style='mso-spacerun:yes'> </span>It’s not
the best buffer for ATP-<span class=SpellE>ase</span>.<o:p></o:p></span></font></li>
</ol>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.25in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Suggestions on better ways will
follow. This is already a lumpy message. .<span
style='mso-spacerun:yes'>
</span><o:p></o:p></span></font></p>
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