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<div class=Section1>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>SSgt Bernie<o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>If this (below) is the same article,
I notice that they have an email given for contact. Maybe ask them the
particulars. <o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>I can tell you that <span
class=SpellE>Trizma</span> HCl and <span class=SpellE>Trizma</span> Base are
from Sigma (look under <span class=SpellE>Trizma</span> in the chemical list). They
supply it like this, in two parts, so you can mix the exact pH buffer you wish
to use (between pH 7 and 9<span class=GramE>) .</span> They give instructions with
the chemicals. Another way to do it is to get <span class=SpellE>Trizma</span>
pre-set crystals which will give you the exact pH you want with out any mixing
of chemicals (between pH 7 and 9).</span></font><o:p></o:p></p>
<p class=MsoNormal><em><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></i></em></p>
<p class=MsoNormal><em><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></i></em></p>
<p class=MsoNormal><em><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></i></em></p>
<p class=MsoNormal><em><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Ann <span class=SpellE>Thorac</span> <span
class=SpellE>Surg</span></span></font></i></em><font size=2><span
style='font-size:10.0pt'> 2000<span class=GramE>;69:84</span>-89<br>
© 2000 <a href="http://ats.ctsnetjournals.org/misc/terms.shtml">The
Society of Thoracic Surgeons</a> </span></font><o:p></o:p></p>
<h3><b><font size=4 face="Times New Roman"><span style='font-size:13.5pt'>
<hr size=1 width="50%" noshade color=gray align=left>
</span></font></b></h3>
<h2><b><font size=5 face="Times New Roman"><span style='font-size:18.0pt'>LV-powered
coronary sinus <span class=SpellE>retroperfusion</span> reduces infarct size in
acutely <span class=SpellE>ischemic</span> pigs <o:p></o:p></span></font></b></h2>
<p class=MsoNormal><strong><nobr></nobr><b><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Jeffrey S. Martin, <span class=SpellE>MD<sup>a</sup></span></nobr>,
<nobr>John G. Byrne, <span class=SpellE>MD<sup>a</sup></span></nobr>, <nobr>Olivier
Y. <span class=SpellE>Ghez</span>, <span class=SpellE>MD<sup>a</sup></span></nobr>,
<span class=SpellE><nobr>Umer</span> <span class=SpellE>Sayeed</span>-Shah, <span
class=SpellE>MD<sup>a</sup></span></nobr>, <nobr>Sergey D. <span class=SpellE>Grachev</span>,
<span class=SpellE>MD<sup>a</sup></span></nobr>, <nobr>Rita G. Laurence, <span
class=SpellE>BS<sup>a</sup></span></nobr>, <nobr>Lawrence H. Cohn, <span
class=SpellE>MD<sup>a</sup></span></nobr> </span></font></b></strong><o:p></o:p></p>
<p><span class=GramE><sup><font size=2 face="Times New Roman"><span
style='font-size:10.0pt'>a</span></font></sup></span><font size=2><span
style='font-size:10.0pt'> Division of Cardiac Surgery, Brigham and
Women's Hospital, </span></font><st1:place><st1:PlaceName><font size=2><span
style='font-size:10.0pt'>Harvard</span></font></st1:PlaceName><font size=2><span
style='font-size:10.0pt'> </span></font><st1:PlaceName><font size=2><span
style='font-size:10.0pt'>Medical</span></font></st1:PlaceName><font size=2><span
style='font-size:10.0pt'> </span></font><st1:PlaceType><font size=2><span
style='font-size:10.0pt'>School</span></font></st1:PlaceType></st1:place><font
size=2><span style='font-size:10.0pt'>, </span></font><st1:place><st1:City><font
size=2><span style='font-size:10.0pt'>Boston</span></font></st1:City><font
size=2><span style='font-size:10.0pt'>, </span></font><st1:State><font size=2><span
style='font-size:10.0pt'>Massachusetts</span></font></st1:State><font size=2><span
style='font-size:10.0pt'>, </span></font><st1:country-region><font size=2><span
style='font-size:10.0pt'>USA</span></font></st1:country-region></st1:place><font
size=2><span style='font-size:10.0pt'> </span></font><o:p></o:p></p>
<p><font size=2 face="Times New Roman"><span style='font-size:10.0pt'>Address
reprint requests to Dr Byrne, Division of Cardiac Surgery, Brigham and
Women's Hospital, </span></font><st1:address><st1:Street><font size=2><span
style='font-size:10.0pt'>75 Francis St</span></font></st1:Street><font
size=2><span style='font-size:10.0pt'>, </span></font><st1:City><font size=2><span
style='font-size:10.0pt'>Boston</span></font></st1:City><font size=2><span
style='font-size:10.0pt'>, </span></font><st1:State><font size=2><span
style='font-size:10.0pt'>MA</span></font></st1:State><font size=2><span
style='font-size:10.0pt'> </span></font><st1:PostalCode><font size=2><span
style='font-size:10.0pt'>02115</span></font></st1:PostalCode></st1:address><br>
<span class=GramE><font size=2><span style='font-size:10.0pt'>e-mail</span></font></span><font
size=2><span style='font-size:10.0pt'>: <a
href="mailto:jgbyrne@bics.bwh.harvard.edu">jgbyrne@bics.bwh.harvard.edu</a></span></font><o:p></o:p></p>
<p><!-- ABS --><span class=GramE><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt;font-style:italic'>Background</span></font></i>.</span>
We developed a prosthetic left ventricle (<st1:City><st1:place>LV</st1:place></st1:City>)
to<sup> </sup>coronary sinus (CS) shunt (LVCSS) that is <span class=SpellE>autoregulating</span>
and<sup> </sup>provides LV-powered retrograde perfusion of the coronary sinus.<sup>
</sup><o:p></o:p></p>
<p><span class=GramE><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt;font-style:italic'>Methods</span></font></i>.</span>
Each of 20 <st1:place>Yorkshire</st1:place> pigs underwent 1 hour of left<sup> </sup>anterior
descending diagonal artery occlusion followed by 3<sup> </sup>hours of
reperfusion. The controls (n = 5) did not have shunt<sup> </sup>treatment. The
LVCSS group (n = 9) underwent shunt treatment<sup> </sup>during the <span
class=SpellE>ischemic</span> period. The LVCSS with partial coronary<sup> </sup>sinus
occlusion (PCSO) group (LVCSS+PCSO, n = 6) underwent shunt<sup> </sup>treatment
and PCSO during the <span class=SpellE>ischemic</span> period. Vital staining<sup>
</sup>and <span class=SpellE>planimetry</span> techniques were used to
determine the area at<sup> </sup>risk for infarction and the area of necrosis.<sup>
</sup><o:p></o:p></p>
<p><span class=GramE><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt;font-style:italic'>Results</span></font></i>.</span>
The area at risk was not significantly different among<sup> </sup>groups. The
area of necrosis was decreased by 53% in the LVCSS<sup> </sup>group and by 73%
in the LVCSS+PCSO group when compared to controls<sup> </sup>(<i><span
style='font-style:italic'>p</span></i> < 0.01 among all groups).<sup> </sup><o:p></o:p></p>
<p><span class=GramE><i><font size=3 face="Times New Roman"><span
style='font-size:12.0pt;font-style:italic'>Conclusions</span></font></i>.</span>
The LVCSS reduces infarct size in pigs after acute<sup> </sup>coronary artery
occlusion. The addition of PCSO to LVCSS further<sup> </sup>improves myocardial
salvage.<sup> </sup><o:p></o:p></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<div>
<p class=MsoAutoSig><font size=3 color=navy face="Courier New"><span
style='font-size:12.0pt;font-family:"Courier New";color:navy;mso-no-proof:yes'>Tim
Morken<o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 color=navy face="Courier New"><span
style='font-size:12.0pt;font-family:"Courier New";color:navy;mso-no-proof:yes'>Lab
Vision / NeoMarkers<o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 color=navy face="Courier New"><span
style='font-size:12.0pt;font-family:"Courier New";color:navy;mso-no-proof:yes'>www.labvision.com<o:p></o:p></span></font></p>
</div>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> Bernard Ian R SSgt 59
CRES/MSROP [mailto:Ian.Bernard@LACKLAND.AF.MIL] <br>
<b><span style='font-weight:bold'>Sent:</span></b> </span></font><st1:date
Month="10" Day="14" Year="2003"><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>Tuesday, October 14, 2003</span></font></st1:date><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> </span></font><st1:time
Hour="7" Minute="2"><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma'>7:02 AM</span></font></st1:time><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'><br>
<b><span style='font-weight:bold'>To:</span></b> Histonet (E-mail)<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] Help; looking
for a stain receipe for TTC<br>
<b><span style='font-weight:bold'>Importance:</span></b> High</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Hello folk or fellow histonetters(in
particular in research), </span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>I'm in the process of
replicating the histochemical staining method in a protocol
article titled " LV-Powered Coronary Sinus Retroperfusion Reduces
infarct Size in Acutely Ischemic Pigs."</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>The made reagents for staining the
heart tissue calls for a pre staining is a Phthalol-blue, which we will be
substituting with Evans Blue. <strong><b><font face=Arial><span
style='font-family:Arial'>I need a concentration and receipe to make this up?</span></font></b></strong> <strong><b><font
face=Arial><span style='font-family:Arial'> The article does not provide
this. </span></font></b></strong> The article states that the "
ascending aorta was occluded and phthalo-blue(Evans Blue) was injected
into the left ventricle.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><strong><b><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Finally the article states</span></font></b></strong><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>: " The
left Ventricle was sliced, parallel to the atriopventricular groove, into
several 5-10 mm slices and incubated for 30 mins at 37 deg C in TTC or
Triphenyltetrazolium Chloride. <strong><b><font face=Arial><span
style='font-family:Arial'>There are 2 concentrations by Sigma, which one is to
be used here, a 95% or 99.5 %? Need receipe to make this solution?</span></font></b></strong></span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><strong><b><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>What is Trisma HCL and Trisma
Base? I need catalog #'s and source?</span></font></b></strong><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><strong><b><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>The article goes on to state for
results</span></font></b></strong><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>:That "the Mycardial tissue
containing dehydrogenase enzymes is stained brick red by TTC, but areas of
necrotic tissue depleted of these enzymes are not stained and appear as white
or pale yellow."</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Thanks</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>SSgt Bernie</span></font><o:p></o:p></p>
</div>
</div>
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