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<DIV><FONT face=Arial size=2>Scott,</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>It would be pointless to perform a PAS and a Jones
silver together as a combination since they both demonstrate the same
elements.</FONT></DIV>
<DIV><FONT face=Arial size=2>Jones silver method for basement membranes, uses
the methenamine silver as an aldehyde detection system in place of the Schiff
reagent used in PAS.</FONT></DIV>
<DIV><FONT face=Arial size=2>The reason for this is to provide a more intense
and contrasty demonstration of the basement membrane in thin sections of kidney.
A PAS stain tends to be too weak in 1-2 micrometer sections.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Regards,</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Bryan</FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=scott.turner@dnax.org href="mailto:scott.turner@dnax.org">Turner,
Scott</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=histonet@pathology.swmed.edu
href="mailto:histonet@pathology.swmed.edu)">HistoNet Server
(histonet@pathology.swmed.edu)</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Monday, October 13, 2003 7:57
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> [Histonet] Jones/PAS</DIV>
<DIV><BR></DIV>
<P><FONT face=Arial size=2>A colleague of mine needs to do a Jones' silver
stain in kidney in combination with PAS. Does anyone have a protocol for
doing these two together?</FONT></P>
<P><FONT face=Arial size=2>Scott Turner</FONT> <BR><FONT face=Arial
size=2>DNAX Research Institute</FONT> <BR><FONT face=Arial size=2>Palo Alto,
CA</FONT> </P><FONT
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