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<DIV><FONT size=2>I've always run one positive control for each antibody and a
negative control for each paraffin block. When I was the immuno supervisor at
AFIP (during another life) we would anywhere from 25-100 cases of the same
antibody, i.e. we would run 1 CD45 control and 50 patient slides, but each
patient slide would have 1 negative. I haven't had a problem yet,
including both the CAP and CLIA inspections I went through this
year.</FONT></DIV>
<DIV><FONT size=2> I have to agree with Patti, since I work in
a reference lab, we don't receive the good cases that a hospital would so we
have to make do with what we can get.</FONT></DIV>
<DIV><FONT size=2></FONT> </DIV>
<DIV><FONT size=2>Joe Nocito BS, HT (ASCP) QIHC<BR>Histology
Manager<BR>Pathology Reference Lab<BR>San Antonio, Texas</FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=ploykasek@phenopath.com href="mailto:ploykasek@phenopath.com">Patti
Loykasek</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=HornHV@archildrens.org
href="mailto:HornHV@archildrens.org">Horn, Hazel V</A> ; <A
title=histonet@pathology.swmed.edu
href="mailto:histonet@pathology.swmed.edu">histonet</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Monday, October 13, 2003 2:18
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Histonet] IHC QC's</DIV>
<DIV><BR></DIV><FONT face="Comic Sans MS">There was in fact, a post from Nick
Kirk on running a positive control with each case. I do realize the CAP
requirements and am familiar with the checklist. <BR><BR>Patti
Loykasek<BR>Phenopath Laboratories<BR>Seattle, WA<BR></FONT>
<BLOCKQUOTE><FONT face="Comic Sans MS"><FONT color=#0000ff><BR><BR>I don't
think anyone said a POSTIVE control should be run with each slide.
We were talking about negative controls, I believe.<BR>I
just copied and pasted from the latest CAP survey in another
email.<BR></FONT></FONT><FONT face=Verdana><BR> <BR><FONT size=2>Hazel
Horn, HT/HTL (ASCP)<BR>Histology Supervisor<BR>Arkansas Children's
Hospital<BR><BR>Phone - 501.364.4240<BR>Fax - 501.364.3912
<BR></FONT></FONT>
<BLOCKQUOTE><FONT face=Verdana><BR></FONT><FONT size=2><FONT
face=Tahoma>-----Original Message-----<BR><B>From:</B> Patti Loykasek
[mailto:ploykasek@phenopath.com] <BR><B>Sent:</B> Monday, October 13, 2003
10:52 AM<BR><B>To:</B> histonet<BR><B>Subject:</B> [Histonet] IHC
QC's<BR><BR></FONT></FONT><FONT face=Verdana>I'm glad that everyone is so
concerned with both negative and positive IHC controls. There is certainly
more than one side to this issue. I will say that I don't think a positive
QC on every slide is absolutely necessary, for many reasons. If the QC is
rare & precious, then it is a waste of resources. As is running a
negative control for every possible technique permutation on small amounts
of tumor. I would rather have slides with tumor left for additional
studies than have wasted tumor sections on 4-6 negative controls. You can
always evaluate non-specific staining on slides that have had an antibody
applied & that are negative with that antibody. The CAP is specific
that positive controls be used for each antibody - see CAP checklist
ANP.22550. They do not specify for each slide. Since positive QC's should
be kept filed for the same number of years as the patient slide &
records, it should be possible to pull a QC slide from the IHC run for a
particular slide. In the CAP comment on ANP.22550, the use of internal
QC's is also mentioned. Although there are many ways of dealing with the
issue of QC's, I'm sure we all want to do what is prudent, abide by the
regulations, and increase the level of patient care. <BR>Just my 2 cents
worth. <BR><BR>Patti Loykasek<BR>Phenopath Laboratories<BR>Seattle, WA
<BR></FONT></BLOCKQUOTE><FONT face=Verdana>
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