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<DIV>If you use a slide adhesive such as sta-on in your waterbath it will cause
a purple haze on the slide if you use too much.</DIV>
<DIV>Dallas</DIV>
<BLOCKQUOTE dir=ltr
style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=nick.kirk3@btopenworld.com
href="mailto:nick.kirk3@btopenworld.com">Nick Kirk</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A
title=histonet@lists.utsouthwestern.edu
href="mailto:histonet@lists.utsouthwestern.edu">Histonet</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Wednesday, October 08, 2003 11:28
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> RE: [Histonet] Purple
Haze.....</DIV>
<DIV><BR></DIV>
<DIV><SPAN class=609172104-09102003><FONT
color=#0000ff>Roxanne</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT
color=#0000ff></FONT></SPAN> </DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>I can't see how the
temperature of the water on the stainer could be causing this artefact as the
problem tends to be intermittent even when batch staining.
</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>Sometimes within a
rack of slides you will get some slides from GI biopsies showing the artefact
while others from the same clinic session do not.</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>It has to be something
individual to each specimen in my opinion, otherwise we would see every slide
going through on that particular batch exhibiting the same staining artefact,
which we don't.</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>I still think the
actual biopsy process has a major part to play, especially as that's the only
variable we don't have any control over and all the others we
do.</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>The temperature thing
is a spurious argument in my opinion when you consider all blocks are chilled
prior to cutting so everything is cold at some point and if you do immuno
antigen retrieval you apply excessive heat to the slide and still get good
nuclear detail when the section is counterstained.</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>I think it has to be
something else.</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT
color=#0000ff></FONT></SPAN> </DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>Nick
Kirk</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT
color=#0000ff>Histopathology</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT color=#0000ff>Hinchingbrooke
Hospital</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT
color=#0000ff>Huntingdon</FONT></SPAN></DIV>
<DIV><SPAN class=609172104-09102003><FONT
color=#0000ff>England</FONT></SPAN></DIV>
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<DIV class=OutlookMessageHeader dir=ltr align=left><FONT
face=Tahoma>-----Original Message-----<BR><B>From:</B>
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]<B>On Behalf Of
</B>GREYTRUNK@aol.com<BR><B>Sent:</B> 09 October 2003 03:29<BR><B>To:</B>
ryaskovich@dir.nidcr.nih.gov; STEGTM@samcstl.org;
KMMerril@LancasterGeneral.org;
histonet@pathology.swmed.edu<BR><B>Subject:</B> Re: [Histonet] Purple
Haze.....<BR><BR></FONT></DIV>
<DIV>Check the temperature of the water on your stainer. </DIV>
<DIV>Roxanne</DIV></BLOCKQUOTE></BLOCKQUOTE></BODY></HTML>