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<DIV><SPAN class=995002221-08102003><FONT face=Arial
color=#0000ff>Jennifer,</FONT></SPAN></DIV>
<DIV><SPAN class=995002221-08102003><FONT face=Arial
color=#0000ff></FONT></SPAN> </DIV>
<DIV><FONT face=Arial><FONT color=#0000ff><SPAN class=995002221-08102003>We cut
levels x 3 on all negative sentinel lymph node blocks. Levels 1 & 3
become H&E's and two #2 levels are held for possible IHC's.
We rough cut into the block approximately 1/3 of it's depth
and pickup the 1st level. The 2nd levels are at mid block and the
third level is at the deeper 2/3's of the block.</SPAN> <SPAN
class=995002221-08102003> I guess in answer to your question about
intervals between levels, here it is determined relative to the depth of
the tissue in the paraffin block.</SPAN></FONT></FONT></DIV>
<DIV><FONT face=Arial><FONT color=#0000ff><SPAN class=995002221-08102003><FONT
face=Arial color=#800000 size=2></FONT></SPAN></FONT></FONT> </DIV>
<DIV><FONT face=Arial><FONT color=#0000ff><SPAN class=995002221-08102003><FONT
face=Arial color=#800000 size=2>Greg Luck</FONT> <BR><FONT face=Arial
color=#808080 size=2>Anatomic Pathology Supervisor</FONT> <BR><FONT face=Arial
color=#800000 size=2>Deaconess Medical Center</FONT> <BR><FONT face=Arial
color=#808080 size=2>800 W. 5th Ave</FONT> <BR><FONT face=Arial color=#800000
size=2>Spokane, WA 99204</FONT> <BR><FONT face=Arial color=#808080
size=2>509.473.7077</FONT> <BR><FONT face=Arial color=#800000
size=2>luckg@empirehealth.org</FONT> </DIV></SPAN></FONT></FONT>
<DIV><SPAN class=995002221-08102003><FONT face=Arial
color=#0000ff></FONT></SPAN> </DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT
face=Tahoma>-----Original Message-----<BR><B>From:</B> JENNIFER SCHUMACHER
[mailto:jschuma1@FAIRVIEW.ORG]<BR><B>Sent:</B> Wednesday, October 08, 2003
1:35 PM<BR><B>To:</B> histonet@lists.utsouthwestern.EDU<BR><B>Subject:</B>
[Histonet] Sentinel nodes<BR><BR></FONT></DIV>
<DIV>Good afternoon everyone,</DIV>
<DIV>We routinely cut our negative sentinel node biopsies shallow, cutting 5
levels (mounting levels 2 and 4 for IHC), approximately 30 microns between
levels. Now, a pathologist read that "most institutions" are cutting
them deeper, approximately 100 microns between levels. This is totally
an INFORMAL poll, but I would appreciate any feedback from the
community. I will check the archives as well, but thought I would get
the question out there before everyone heads off to Kentucky:-)
Thanks. Jennifer</DIV></BLOCKQUOTE></BODY></HTML>