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<DIV>When I H&E my frozen sections I don't use Xylene. I merely skip
the Xylene and alcohol steps, go straight to dH2O and stain. When I get to
Eosin, I just rinse with dH2O and mount coverslips on (if possible) with
ageous mounting media. This is probably no help if you have no way to
coverslip them. But, this is my suggestion.</DIV>
<DIV>Gareth</DIV>
<DIV> </DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
<DIV> </DIV>
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<DIV><FONT size=2>-----Original Message----- <BR><B>From:</B> Johnson, Kevin
[mailto:KJohnson@med.miami.edu] <BR><B>Sent:</B> Fri 10/3/2003 4:29 PM
<BR><B>To:</B> 'histonet@lists.utsouthwestern.edu' <BR><B>Cc:</B>
<BR><B>Subject:</B> [Histonet] Cell Culture Staining<BR><BR></FONT></DIV>
<P><FONT size=2>Greetings, all.<BR><BR>A researcher just brought me (at Miller
Time on a Friday!) two culture<BR>flasks of terminally differentiated Sertoli
cells and requested H&E<BR>staining. Well. <BR><BR>Conventional
H&E obviously is out, since polystyrene is not compatible
with<BR>xylene. Further restrictions: the cells cannot be scraped off,
they cannot<BR>be replated on glass coverslips, and for reasons of her own,
they cannot go<BR>back into the incubator pending an answer. They now
have been fixed in situ<BR>with 10% neutral buffered formalin.<BR><BR>Does
anyone have a protocol for staining these puppies? If not H&E per
se,<BR>then is there another staining method that might satisfy
her?<BR><BR>Thanks in advance,<BR><BR>Kevin Johnson<BR>University of
Miami<BR>Diabetes Research
Institute<BR><BR><BR>_______________________________________________<BR>Histonet
mailing list<BR>Histonet@lists.utsouthwestern.edu<BR><A
href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet">http://lists.utsouthwestern.edu/mailman/listinfo/histonet</A><BR></FONT></P></BLOCKQUOTE>
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