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<DIV>Hi, membrane slides are like plastic wrap over a glass slide, hand glued or
you can buy them in a hard plastic cut-out slide with the plastic membrane
sealed into it. I'm not sure you can charge the membrane but I don't lay it on
the water bath. It seems that the 12 microns that the researcher wanted it cut
at was too thick. I have since found out it should be cut at 6 microns for
laser cutting and DNA studies. But I will keep your info in mind when I cut with
charged slides. Thanks for the tip.</DIV>
<DIV>Lil Muolo</DIV>
<DIV>Immuno. Lab</DIV>
<DIV>Cancer Institute of New Jersey<BR><BR>>>> Margaret Horne
<mhorne@upei.ca> 10/1/03 10:45:20 AM >>><BR></DIV>
<DIV>I don't know about membrane slides , but I had a lot of problems <BR>with
tissue floating off during IHC. Then someone pointed out that I <BR>was using
charged slides but laying them out on the side of the <BR>water bath. The water
bath is grounded and therefore I may have <BR>been losing the charge on the
slide and it's the charge that keeps <BR>ithe tissue on. Stopped touching the
slides to the water bath and <BR>stopped losing tissue.What can I say... it
worked for me. <BR>Membrane slides may be very different. I don't know.
<BR><BR> Good
luck,<BR><BR>
Margaret<BR>Margaret Horne ,<BR>Histology Teaching Assistant,<BR>Dept. of
B.SC.,<BR>Atlantic Veterinary College, U.P.E.I.,<BR>550 University Ave.,
Charlottetown,<BR>P.E.I., C1A 4P3 <BR>Canada<BR><BR></DIV></BODY></HTML>