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<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff
size=2>Jesus,</FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff size=2>I
can't recall a single lab that we introduced our MONOCLONAL kappa and
lambda to that didn't solve their background problem, especially with bone
marrows. We have plenty of clients that use our monoclonal kappa and
lambda on a variety of stainers, and we have found that often times heat
retrieval with EDTA gives cleaner results than enzyme retrieval. I can
supply you with references if needed.</FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff size=2><A
href="http://www.cellmarque.com/2000/Pages/catalog/kappa.html">http://www.cellmarque.com/2000/Pages/catalog/kappa.html</A></FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003></SPAN> </DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff size=2><A
href="http://www.cellmarque.com/2000/Pages/catalog/lambda.html">http://www.cellmarque.com/2000/Pages/catalog/lambda.html</A></FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff size=2>Jeff
Gordon</FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff
size=2>Domestic Technical Sales and Marketing Manager</FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff size=2>Cell
Marque Corp.</FONT></SPAN></DIV>
<DIV><SPAN class=120374914-01102003><FONT face=Arial color=#0000ff
size=2>1-800-665-7284, Ext. 12</FONT></SPAN></DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Jesus Ellin
[mailto:Ellin13@adelphia.net]<BR><B>Sent:</B> Tuesday, September 30, 2003 7:00
PM<BR><B>To:</B> histonet@lists.utsouthwestern.edu<BR><B>Subject:</B>
[Histonet] Kappa, Lambda staining???<BR><BR></FONT></DIV>
<DIV><CODE><FONT size=3><FONT face=Arial size=2>Hello everyone ,, Need some
help here, currently our lab is trying to create a IHC protocol for kappa and
lambda on the Venntana benchmark,, we are having some real problems with the
background staining in our control and patients,, we are using a blocker and
or hydrogen peroxide, to try and minimize the peroxidase but we are still
getting alot of background, if any one out there that has a benchmark
and has a protocol for these stains some help would be appreciated,, forgot to
mention that we are using Tonsil and Bone Marrow for
control,</FONT></FONT></CODE></DIV>
<DIV><CODE><FONT size=3><FONT face=Arial
size=2></FONT></FONT></CODE> </DIV>
<DIV><CODE><FONT size=3><FONT face=Arial size=2>One other thing how many out
there are using the Tamtron System,, we are currently in the building phase
and would like to ask some people out there questions concerning proccess,
codes and end user training</FONT></FONT></CODE></DIV>
<DIV><CODE><FONT size=3><FONT face=Arial
size=2></FONT></FONT></CODE> </DIV>
<DIV><CODE><FONT size=3><FONT face=Arial
size=2></FONT></FONT></CODE> </DIV>
<DIV><CODE><FONT size=3><FONT face=Arial size=2>Jesus Ellin HTL
ASCP </FONT></FONT></CODE></DIV>
<DIV><CODE><FONT size=3><FONT face=Arial size=2>Yuma Regional Medical
Center</FONT></FONT></CODE></DIV>
<DIV><CODE><FONT size=3><FONT face=Arial
size=2>jellin@yumaregional.org</FONT></DIV></BLOCKQUOTE></FONT></CODE></BODY></HTML>