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<P><FONT size=2>Hi Liz,<BR><BR>FLK-1 and FLT-1 are cell membrane receptors for
VEGF; Fli-1 is a transcription factor/regulator. As it is a nuclear protein,
yes, in a strict sense, it doesn't stain the same as CD31 or CD34 or Factor
VIII, but it is specific for endothelial cells.<BR></FONT><FONT
size=2>Ronnie</FONT></P>
<P><FONT size=2><EM><FONT face=Arial color=#0000ff size=1>Ronnie Houston</FONT>
<BR><FONT face=Arial color=#0000ff size=1>Regional Histology Operations
Manager</FONT> <BR><FONT face=Arial color=#0000ff size=1>Bon Secours
HealthPartners Laboratories</FONT> <BR><FONT face=Arial color=#0000ff
size=1>5801 Bremo Road</FONT> <BR><FONT face=Arial color=#0000ff
size=1>Richmond, VA 23226</FONT> <BR><FONT face=Arial color=#0000ff size=1>(804)
287 7972</FONT> <BR><FONT face=Arial color=#0000ff
size=1>ronnie_houston@bshsi.com</FONT></EM> </P>
<P>Hi Liz,<BR><BR>-----Original Message-----<BR>From: Elizabeth Chlipala [<A
href="mailto:lizchlipala@premierhistology.com">mailto:lizchlipala@premierhistology.com</A>]<BR>Sent:
Thursday, September 25, 2003 10:58 AM<BR>To: 'Fearn Tony';
histonet@lists.utsouthwestern.edu<BR>Subject: RE: [Histonet]
FLI-1<BR><BR><BR>Tony<BR><BR>I believe the antibody you are talking about is
FLK-1 or FLT-1, another<BR>name for it is VEGF-R2. From what I understand
it does not stain the<BR>same as CD34, I don't believe it is specific for
endothelial cells, it<BR>will stain other things also. You can get this
antibody from Santa<BR>Cruz. We have worked on this in the past and in our
hands it was a<BR>tough one. It does play a role in
angiogenesis.<BR><BR>Liz <BR><BR>Elizabeth A. Chlipala, BS,
HTL(ASCP)<BR>Premier Histology Laboratory, LLC<BR>P.O. Box 18592<BR>Boulder,
Colorado 80308<BR>Office: (303) 735-5001<BR>Fax: (303)
735-3540<BR>lizchlipala@premierhistology.com<BR>www.premierhistology.com<BR><BR>Ship
to Address:<BR>Premier Histology Laboratory<BR>University of Colorado<BR>MCBD,
Room A3B40<BR>Boulder, Colorado
80309<BR><BR>_________________________________<BR>This email and any files
transmitted with it may contain PRIVILEGED or<BR>CONFIDENTIAL information and
may be read or used only by the intended<BR>recipient. If you are not the
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that you have received this email in<BR>error and that any use, dissemination,
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reply email or<BR>contact the sender at the number listed above if one is
provided.<BR><BR><BR>-----Original Message-----<BR>From:
histonet-admin@lists.utsouthwestern.edu<BR>[<A
href="mailto:histonet-admin@lists.utsouthwestern.edu">mailto:histonet-admin@lists.utsouthwestern.edu</A>]
On Behalf Of Fearn Tony<BR>Sent: Thursday, September 25, 2003 5:15 AM<BR>To:
'histonet@lists.utsouthwestern.edu'<BR>Subject: [Histonet]
FLI-1<BR><BR><BR><BR>> One of our pathologists has just returned from a
conference in Milan<BR>and<BR>> is asking if we can get FLI-1, as an
endothelial marker, that is meant<BR>to<BR>> be better than CD34. I've looked
in the DakoCytomation and Novocastra<BR>> catalogues but there is no mention
of it. Does anyone know where I can<BR>get<BR>> it, and is it worth
buying?<BR>><BR>> Anthony J Fearn<BR>> Chief Biomedical
Scientist<BR>>
Histopathology<BR>><BR>><BR><BR>_______________________________________________<BR>Histonet
mailing list<BR>Histonet@lists.utsouthwestern.edu<BR><A
href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
target=_blank>http://lists.utsouthwestern.edu/mailman/listinfo/histonet</A><BR><BR><BR>_______________________________________________<BR>Histonet
mailing list<BR>Histonet@lists.utsouthwestern.edu<BR><A
href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
target=_blank>http://lists.utsouthwestern.edu/mailman/listinfo/histonet</A><BR></P></FONT></BODY></HTML>
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