<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=us-ascii">
<meta name=ProgId content=Word.Document>
<meta name=Generator content="Microsoft Word 10">
<meta name=Originator content="Microsoft Word 10">
<link rel=File-List href="cid:filelist.xml@01C383AE.0B1D8CE0">
<!--[if gte mso 9]><xml>
<o:OfficeDocumentSettings>
<o:DoNotRelyOnCSS/>
</o:OfficeDocumentSettings>
</xml><![endif]--><!--[if gte mso 9]><xml>
<w:WordDocument>
<w:SpellingState>Clean</w:SpellingState>
<w:GrammarState>Clean</w:GrammarState>
<w:DocumentKind>DocumentEmail</w:DocumentKind>
<w:EnvelopeVis/>
<w:Compatibility>
<w:BreakWrappedTables/>
<w:SnapToGridInCell/>
<w:WrapTextWithPunct/>
<w:UseAsianBreakRules/>
</w:Compatibility>
<w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel>
</w:WordDocument>
</xml><![endif]-->
<style>
<!--
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-parent:"";
margin:0in;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";}
a:link, span.MsoHyperlink
{color:blue;
text-decoration:underline;
text-underline:single;}
a:visited, span.MsoHyperlinkFollowed
{color:purple;
text-decoration:underline;
text-underline:single;}
span.EmailStyle17
{mso-style-type:personal-compose;
mso-style-noshow:yes;
mso-ansi-font-size:10.0pt;
mso-bidi-font-size:10.0pt;
font-family:Arial;
mso-ascii-font-family:Arial;
mso-hansi-font-family:Arial;
mso-bidi-font-family:Arial;
color:windowtext;}
span.SpellE
{mso-style-name:"";
mso-spl-e:yes;}
span.GramE
{mso-style-name:"";
mso-gram-e:yes;}
@page Section1
{size:8.5in 11.0in;
margin:1.0in 1.25in 1.0in 1.25in;
mso-header-margin:.5in;
mso-footer-margin:.5in;
mso-paper-source:0;}
div.Section1
{page:Section1;}
-->
</style>
<!--[if gte mso 10]>
<style>
/* Style Definitions */
table.MsoNormalTable
{mso-style-name:"Table Normal";
mso-tstyle-rowband-size:0;
mso-tstyle-colband-size:0;
mso-style-noshow:yes;
mso-style-parent:"";
mso-padding-alt:0in 5.4pt 0in 5.4pt;
mso-para-margin:0in;
mso-para-margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:10.0pt;
font-family:"Times New Roman";}
</style>
<![endif]-->
</head>
<body lang=EN-US link=blue vlink=purple style='tab-interval:.5in'>
<div class=Section1>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>PARDON <span class=SpellE>PARDON</span> <span class=SpellE>PARDON</span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I left out a page of the Nerve and Muscle procedures when I
sent them out. It is a method that keeps the nerve tissue sections on the slide
during silver impregnations. Silver nitrate is notorious for loosening sections
on a slide.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>The preparation uses <span class=SpellE>Claoue’s</span>
Cement--- adapted from a process in The <span class=SpellE>Microtomist’s</span>
Formulary and Guide by Peter Gray, from the <span class=SpellE>Blakiston</span>
<span class=SpellE>Co.i<i style='mso-bidi-font-style:normal'><span
style='font-style:italic;mso-bidi-font-style:normal'>nc</span></i></span><i
style='mso-bidi-font-style:normal'><span style='font-style:italic;mso-bidi-font-style:
normal'><o:p></o:p></span></i></span></font></p>
<p class=MsoNormal><i style='mso-bidi-font-style:normal'><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial;font-style:italic;
mso-bidi-font-style:normal'><o:p> </o:p></span></font></i></p>
<p class=MsoNormal><span class=GramE><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>It’s</span></font></span><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> main
ingredient:<span style='mso-spacerun:yes'> </span><span
class=SpellE>Collodion</span>---<span class=SpellE>Mallinkrodt</span> order
number UN 1993<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>FORMULA:<o:p></o:p></span></font></p>
<p class=MsoNormal><span class=SpellE><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Claoue’s</span></font></span><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> Cement:<o:p></o:p></span></font></p>
<p class=MsoNormal><span class=SpellE><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Collodion</span></font></span><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><span
style='mso-spacerun:yes'>
</span>8 ml<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>100% Ethyl <span class=GramE>Alcohol <span
style='mso-spacerun:yes'> </span>92</span> ml<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Camphor<span
style='mso-spacerun:yes'>
</span>2 gm<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Castor Oil<span
style='mso-spacerun:yes'>
</span>3 ml<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>PROCEDURE:<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I usually mixed 2 or 3 times the above amounts to make 200
or 300 ml of <span class=SpellE>Claoue’s</span>. Kept tightly closed, it
keeps for a long time.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I know this looks like snake oil, but it works.<span
style='mso-spacerun:yes'> </span>Regular <span class=SpellE>pyroxylin</span>
or <span class=SpellE>celloidin</span> solutions in alcohol and ether <span
class=GramE>alone,</span> can form a kind of sheet which can peel off taking
the tissues with it.<span style='mso-spacerun:yes'> </span>This prep
forms an emulsion which keeps the tissues on the slides very nicely.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Paraffin Sections:<span style='mso-spacerun:yes'>
</span>Remove paraffin as usual.<span style='mso-spacerun:yes'>
</span>After the last 95% alcohol, remove one or two slides from the alcohol to
put on a clean paper towel. Place two or three sheets of bibulous paper very
carefully on the slides.<span style='mso-spacerun:yes'> </span>Blot
gently with two or three fingers<span style='mso-spacerun:yes'>
</span><o:p></o:p></span></font></p>
<p class=MsoNormal><span class=GramE><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>for</span></font></span><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> two or
three strokes from one end of the slide to the other. Carefully turn the
bibulous paper over and repeat the blotting as before. This smoothes wrinkles
and fixes the tissue to the slide. With an eye dropper, apply enough drops of <span
class=SpellE>Claoue’s</span> to cover the sections and to the edge of the
slide where the sections are. Drain any surplus <span class=SpellE>Claoue’s</span>
off by touching both edges of the slides to the bibulous paper. Blow gently on
the slides until the <span class=SpellE>Claoue’s</span> turns opaque.
Rinse in two changes of 95% ethyl alcohol and proceed with what ever stain you
are doing. This preparation is compatible with silver impregnations and the <span
class=SpellE>Kluver-Berrera</span> beautiful <span class=SpellE>luxol</span>
fast blue and <span class=SpellE>cresyl</span> fast violet---and many others.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>When the silver impregnation is done, the slides are run up
the alcohols. In the first two 100% alcohols, the <span class=SpellE>Claoue’s</span>
coating will come off with some silver giving it color. After two more 100%
alcohols, clear and cover as usual. Be careful in these final stages.<span
style='mso-spacerun:yes'> </span>With the <span class=SpellE>Claoue’s</span>
covering removed, the sections may loosen if handled roughly.<span
style='mso-spacerun:yes'> </span>I say this, although I have never lost a
section at this time in the procedure.<span style='mso-spacerun:yes'>
</span>But I’m always careful there anyway.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Good luck with your silver preps.<span
style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><span style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><span style='mso-spacerun:yes'> </span><o:p></o:p></span></font></p>
</div>
</body>
</html>