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<DIV><FONT color=#000080>Colleen,</FONT></DIV>
<DIV><FONT color=#000080>I have question for you on your c-fos
protocol. </FONT></DIV>
<DIV><FONT
color=#000080>
"I do a steam retrievel using a 6.0pH buffer for 30 minutes<BR>followed by a 10
minute cool down"</FONT></DIV>
<DIV><FONT color=#000080>Exactly what buffer do you use and how do you go about
the steam retrievel? I used to place my coplin jar (with slides and
buffer) in a beaker of boiling water - is that what you do?</FONT></DIV>
<DIV><FONT color=#000080>Thanks,</FONT></DIV>
<DIV><FONT color=#000080>Gareth</FONT></DIV>
<DIV> </DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
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