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<br>
Stanley.Lupo@GSK.Com<br>
Safety Assessment<br>
Mail Code: UE0461<br>
Phone: 610 270-7340<br>
Fax: 610 270-7202</font>
<br><font size=2 face="Arial">Fellow Histonetter, </font>
<br><font size=2 face="Arial">In our lab, proper orientation begins at prosection. The mesentery is laid out flat, then rolled around the rubber plunger of a 1 mL syringe. The button that is formed when the rolled mesentery is removed from the plunger is placed in a plastic capsule and fixed in 10% Neutral Buffered Formalin.</font>
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<br><font size=2 face="Arial">Processing involves 40 minute cycles through 95% EtOH, then 60 minute cycles through 100% EtOH, 40 minutes each through 2 changes of toluene, followed by 4 changes of paraffin at 150 minutes (total). Total processing time is 9 hours.</font>
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<br><font size=2 face="Arial">The mesenteric roll is embedded on edge, and care is taken not to oversoak the block.</font>
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<br><font size=2 face="Arial">Good luck.</font>
<br>
<br><font size=2 face="Arial">Stan Lupo</font>
<br><font size=2 face="Arial">Department of Safety Assessment</font>
<br><font size=2 face="Arial">GlaxoSmithKline</font>
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<td><font size=1 face="sans-serif"><b>Felix.Rintelen@serono.com</b></font>
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<br><font size=1 face="sans-serif">Sent by: histonet-admin@lists.utsouthwestern.edu</font>
<p><font size=1 face="sans-serif">18-Sep-2003 04:18</font>
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<br><font size=1 face="sans-serif"> To: Histonet</font>
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<br><font size=1 face="sans-serif"> cc: </font>
<br><font size=1 face="sans-serif"> Subject: [Histonet] how to embed/section mesentery?</font></table>
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<br><font size=2 face="Courier New">Dear all,<br>
<br>
Is there anybody who may give me advice how to make good sections of<br>
parafin embedded mesentery (mouse)? How to prepare the tissue for embedding<br>
and how to handle it to embed/orient it properly in the parafin block? My<br>
concern is especially the soft consistency of the tissue making me feel<br>
that the proper orientation in the block might be difficult.<br>
<br>
Thank you very much in advance,<br>
<br>
Felix<br>
<br>
Felix Rintelen<br>
Serono Pharmaceutical Research Institute<br>
Geneva, Switzerland<br>
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