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<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>Clarification,</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>Thanks for all your responses. Its clear that I
haven't explained myself very well at all:</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>We would like to use oil red O to quantify lipid
localisation in frozen sections of hepatic and muscle tissue. However, we
must first demonstrate that the amount of staining we get (density) is in fact equal
to the amount of lipid present in the sections and to do this we need to set up
some titrations using positive controls with known and different concentrations
of lipid to show a linear response between staining intensity and lipid
concentration. However, a fat smear is difficult to use as a positive
control in this way because it simply washes off the slides. Therefore,
we would like to know what others have done to demonstrate linearity with the
oil red O staining procedure. Is there a way we can treat the fat to
overcome its liquid nature (that is to say not the lipid in the muscle and
liver but the actual 'fat' being used as a positive control)?</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>Thanks</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>Jeremy</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'> </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>Jeremy Browne </span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>Liggins Institute</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'>University of Auckland</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>NZ (09) 373 7599 extn 86444</span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=EN-AU style='font-size:
10.0pt;font-family:Arial'><a href="mailto:j.browne@auckland.ac.nz"><span
lang=PT-BR>j.browne@auckland.ac.nz</span></a></span></font></p>
<p class=MsoNormal><font size=1 face="Times New Roman"><span lang=PT-BR
style='font-size:8.0pt'> </span></font></p>
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