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I am attempting to use Masson's trichrome stain on some rat bone marrow
stem cells cultured on fibrous contstructs. Following staining with
the acid fuchsin, a large amount of tissue growing in between the
fibers is staining red. Following the treatment with aniline blue,
however, this tissue is no longer visible. In some areas it appears as
though it has torn away from the fibers where it was previously spread
between (some arease of floating tissue are visible). My initial
thoughts were that perhaps this tissue was muscle, although this was a
bit supprising as these cells should be differentiating into
osteoblasts in the presence of these fibers. I had also read somewhere
that the collagen would stain red with acid fuchsin, however,
subsequent staining with aniline blue should result in collagen
appearing blue. The only problem is I am seeing neither blue nor red
cells in these specific areas following aniline blue staining. Any
suggestions???<br>
Thanks<br>
Brian<br>
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Brian Hatcher<br>
Graduate Research Assistant <br>
Department of Biomedical Engineering<br>
University of Florida<br>
PO Box 116400<br>
Gainesville, FL 32611-6400<br>
Ph: 352-392-6656<br>
Fax: 352-392-3771<br>
email: <a class="moz-txt-link-abbreviated" href="mailto:briany@ufl.edu">briany@ufl.edu</a><br>
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