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<DIV><FONT face=Arial size=2>Dear All,</FONT></DIV>
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<DIV><FONT face=Arial size=2>I am currently trying to detect the existence of
different muscarinic receptor subtypes in human tissues using
immunofluorescence. My samples are OCT imbedded. I have tried
fixation with 4% paraformaldehyde, acetone, acetone:methanol, 95% ethanol,
neutral buffered formalin. I have used primary antibodies from R &
D and Biogenesis. So far all the antibodies light up the whole
cell with no intensified staining on the plasma membrane.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>I wonder if any one has done stainings on
muscarinic receptors or other 7 transmembrane protein receptors and like to
share your success with me.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Thank you very much in advance.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Andea Liu</FONT></DIV>
<DIV><FONT face=Arial size=2>Ph D student</FONT></DIV></BODY></HTML>