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<DIV>I plan to do a immunohistochemical stain on mouse brain tissue with
c-fos. Some protocols I've read state that you should osmicate the
sections, after mounting, to enhance the stain. I've never stained with
c-fos, but I have been told it is very dark on it's own. Does anyone
know if it will be necessary to osmicate. Of course, I guess I could just
try and see, but any information on the staining process itself would be
helpful.</DIV>
<DIV>Thanks,</DIV>
<DIV>Ms. Gareth Davis</DIV>
<DIV>Vanderbilt University Medical Center</DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
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