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<DIV><FONT size=2>Gareth,<BR><BR>I am not doing free floating sections, mine are
cut at 5u and mounted on<BR>glass. I do a steam retrievel using a 6.0pH buffer
for 30 minutes<BR>followed by a 10 minute cool down. Then I rinse well, buffer
rinse for<BR>5-10 minutes and stain. I use the c-fos from Oncogene,Cat#PC38
at<BR>1:20,000 overnight at 4 degrees followed by Signet
biotinylated<BR>secondary and streptavidin third and DAB as a chromagen. I
counter stain<BR>1 min with Haematoxylin and run down and coverslip.<BR><BR>Hope
this helps.<BR><BR>Colleen Forster<BR>U of MN</FONT><BR></DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
<DIV> </DIV>
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