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<DIV><SPAN class=196173013-27082003><FONT face=Georgia>I had this happen
repeatedly and eventually found out the rotator was bad on the processor so be
aware of further problems. The rotator on my processor would rotate at will and
plunk in any basket. </FONT></SPAN></DIV>
<DIV><SPAN class=196173013-27082003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=196173013-27082003><FONT face=Georgia>To rescue the tissue I
would just place back in the solution it was in before suspended in air then
proceed with the processing. I would mostly with mouse tissue and samples were
readable but we elected to repeat our studies as the morphology was less than
perfect and cutting was a nightmare. Not hopeless just
difficult!</FONT></SPAN></DIV>
<DIV><SPAN class=196173013-27082003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=196173013-27082003><FONT
face=Georgia></FONT></SPAN> </DIV>
<DIV><SPAN class=196173013-27082003><FONT face=Georgia>C</FONT></SPAN></DIV>
<DIV> </DIV>
<P><FONT face="BernhardFashion BT" color=#000080>Cynthia Favara</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>NIAID/NIH/RML/LPVD</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>903 South 4th Street</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>Hamilton, MT 59840</FONT> <BR><FONT
face="BernhardFashion BT" color=#000080>406-363-9317</FONT> </P>
<BLOCKQUOTE>
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Jackie.O'Connor@abbott.com
[mailto:Jackie.O'Connor@abbott.com]<BR><B>Sent:</B> Wednesday, August 27, 2003
6:28 AM<BR><B>To:</B> histonet@lists.utsouthwestern.edu<BR><B>Subject:</B>
[Histonet] Processor failure<BR><BR></FONT></DIV><BR><FONT face=Arial
size=2>Fellow histonetters:</FONT> <BR><FONT face=Arial size=2>I haven't done
this in years. I came in this a.m. to find the tissues on my processor
suspended in air over the first absolute alcohol station. Apparently,
the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck
as it tried to rotate. I have about 100 xenografts to rescue.
Right now they all look and feel like bb's. My plan is to
rehydrate them and reprocess them, but I have my doubts as to the quality of
the morphology and any subsequent IHC. As I said, I haven't screwed up a
basket of tissue in years and years - so I would appreciate any new ideas on
possibly saving these tumors. Thanks in advance.</FONT> <BR><BR><FONT
face=Arial size=2>Jackie O'Connor</FONT></BLOCKQUOTE></BODY></HTML>