[Histonet] Retic Poll

[Alonso Martínez Canabal]

Good morning from Mexico City,
         I am working on a Golgi-Cox staining for mouse brains. The
impregnation of neurons is very good, but I could use more neurons. My main
problem is that I see many blood vessels, which makes it very difficult to
trace the neurons. I also have many astrocytes. I have tried fresh tissue
impregnation, saline perfusion and even formalin perfusion, but the same
results keep appearing.
     Thank you a lot for your advice.


El vie, 6 mar 2026 a las 1:35, Gudrun Lang via Histonet (<
histonet at lists.utsouthwestern.edu>) escribió:

> Hi!
> We used to use Gömöri's technique with in-house-made reagens.
> Today we stain with an automated stainer.
>
> Gudrun
>
> Biomedizinische Analytikerin
> Linz, Österreich
>
>
>
> -----Ursprüngliche Nachricht-----
> Von: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Gesendet: Mittwoch, 4. März 2026 20:47
> An: histonet at lists.utsouthwestern.edu
> Betreff: [Histonet] Retic Poll
>
> Hi Techs - For those of you performing a Manual Retic Stain -
> What method do you use?  And do you use a kit?  Or make your own regent
> components?
> Thanks, in advance, for the information.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> HNL Laboratoy Medicine for
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3689, Fax: 215-938-2021
>           Honesty
> AccouNtability
>     AgiLity
>     CoLlaboration
>   CoMpassion
>
>
>
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-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833