[Histonet] Help to: Changing Alcohol Vendors. Histonet Digest, Vol 261, Issue 3

[Eddie Martin]

>
> Good evening,
>
>
>
> At the end of the day, your SOP should spell out what to do in this
> situation. Let that document, signed off by your technical manager or
> director, guide the call.
>
> Here’s my take (strictly my opinion, not your lab’s):
>
> If you’re using a pure solvent like ethanol or isopropanol, or Xylene.
> the CAS number doesn’t change by vendor.
>
> 1.      If the reagent is part of the stain’s analytical method: (H&E
> stain), then a small comparison study is usually all that’s needed since
> the reagents being switched are exactly the same in their properties &
> concentrations.  The only change is the distributor/vendor the reagent is
> being acquired from.
>
> 2.      If the reagent is *Not* part of the stain’s analytical method:
>  If the alcohol and xylene aren’t method steps for performing the test
> (such as being the dehydration and clearing steps only) then I wouldn’t
> consider that revalidation-worthy.
>
>
>
> I hope this helps!
>
>
>
> Best regards,
> Eddie Martin
> Technical Pathology Specialist
> The National Institutes of Health
> 10 Center Drive
> Building 10, RM 2C301
> Bethesda, MD 20816
> (301) 594-2054
> eddie.martin at nih.gov
>




> On Wed, Jul 23, 2025, 11:44 AM Pairan, Kelly via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > Good Morning,
> > Our hospital is looking at switching our 100% alcohol and 95% alcohol to
> > another vendor.  I have received mixed answers on whether or not we are
> > going to need to a validation for this reagent swap when it comes to
> > processing, staining and IHC.  What is the practice at your institution?
> >
> > Thanks,
> > Kelly
> >
> > Kelly Pairan, HT(ASCP)CM, HQIPCM
> > Technical Scientist Anatomic Pathology and Cytology
> > OhioHealth Laboratory Systems
> > Suite 210 North Medical Building
> > 3535 Olentangy River Rd
> > Columbus, OH 43214
> >
> > Email: kelly.pairan at ohiohealth.com<mailto:kelly.pairan at ohiohealth.com>
> > Work: (614) 566-3575
> > Cell: (614) 312-0104
> >
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>
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