[Histonet] Pentachrome stain questions

[Colleen Forster]

Bernice,

I am aware of this. That is the question.

If in fact, you need that solution to transform the Alcian Blue into a permanent state, how can it work with protocols that don't require that step?

I used the Carson protocol because I was trying several to find one that kept the blue. But I want to understand the "why" here. When you are doing these 100 slides at a time you need it to be reliable and I want to understand.

Colleen Forster
________________________________
From: Bernice Frederick via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, May 22, 2025 2:22 PM
To: Gudrun Lang <gu.lang at gmx.at>; 'Colleen Forster' <cforster at umn.edu>
Cc: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Pentachrome stain questions

[Caution: This email originated from outside the organization.]


I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know.
Bernice

-----Original Message-----
From: Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, May 22, 2025 12:59 PM
To: 'Colleen Forster' <cforster at umn.edu>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Pentachrome stain questions

Hi Colleen,
can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help.

Gudrun

-----Ursprüngliche Nachricht-----
Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu]

Gesendet: Mittwoch, 21. Mai 2025 18:51
An: histonet-request
Betreff: [Histonet] Pentachrome stain questions

Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain.

Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all.

Can some please help me understand these 3 questions:

   1. What is the purpose of this solution?
   2. Is heat only to accelerate the process or necessary?
   3. How can other protocols not use it at all?

I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~
--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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