[Histonet] staining over IHC stained slide

[Amos Brooks]

Hi,
     I do this frequently. It works great with some antibodies, but I have
found some are less than cooperative about it.
     If you are concerned about it, and it would make sense if you were, it
would be a good idea to take the control slide that was used and try it on
that before doing it on patient test tissue.
    As I say thoi it usually works fine. Give it a try.

Amos

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> Message: 1
> Date: Tue, 6 May 2025 20:29:44 +0000
> From: "Olson, Jennifer (Carris Lab)" <Jennifer.Olson at centracare.com>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] staining over IHC stained slide
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> I have a pathologist who is inquiring about staining over and already IHC
> stained slide. He would like to stain over using a different antibody that
> has been stained. He doesn't have a specific case or antibody, he is just
> wondering if there are any histology labs out there that do this?
>
> Any suggestions?
>
> Thank you -
>
>