[Histonet] staining over IHC stained slide

[Obregon, Cecilia]

I do not recommend this. Staining over an already stained IHC can compromise both antigen integrity and staining quality due to previous antigen retrieval. 
We have however done this on previously stained H&E,  just keep in mind you might have to run a modified protocol with 'no-depar' and run a separate slide with a positive control. 

Thank you

Cecilia M. Obregon


-----Original Message-----
From: Olson, Jennifer (Carris Lab) via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Tuesday, May 6, 2025 4:30 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] staining over IHC stained slide

 

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I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this?

Any suggestions?

Thank you -
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