[Histonet] Histonet Digest, Vol 257, Issue 11

[Eddie Martin]

Hi John,

A few common scenarios of why recoverslipping would be needed may give you
the better response you're searching for.  An example may be that the lab
is diluting their coverslip medium too much and slides look great for a few
days, or another scenario, where they forget to prime the tubing in their
instruments or the viscosity is too thick, causing air to get
dispensed rather than coverslipping medium.

Instead of a re-coverslipping percentage, what information are you looking
for?

Best wishes.

Eddie Martin
National Institutes of Health


On Tue, Apr 29, 2025 at 1:00 PM <histonet-request at lists.utsouthwestern.edu>
wrote:

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>    1. Removing H&E coverslippped slide (John O?Brien)
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> ---------- Forwarded message ----------
> From: "John O’Brien" <john at imebinc.com>
> To: histonet at lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Mon, 28 Apr 2025 11:50:40 -0700
> Subject: [Histonet] Removing H&E coverslippped slide
>
> Histonetters
>    Can you experts in staining and Coverslipping offer the number of times
> or % of slides recover slipped to a differ stain, any opinion is
> appreciated
> John
> IMEB Inc
>
>
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