[Histonet] Formic Acid treatment for b-Amyloid staining

[Carl Hobbs]

Hi
If HIER works best for you, stay with it
It is good to do an initial comparison, imho
I find that some anti A-beta abs work better after Formic acid AR, others better after Citric acid HIER
Some anti A-beta abs also disclose intracellular APP after Citra HIER....never so after Formic acid
( eg: anti Amyloid beta 1-17 Abcam ab11132 will show amyloid plaques and intracellular APP after Citra HIER....after Formic acid AR, only amyloid plaques are seen)
Have to admit to having included Congo red (Direct red 28) occasionally....stains the smallest deposits; espec. when including fluorescence examination
Always most interested to read of your Xp, Cathy
NB: I can't open  Bonnie's link so, can't comment


Best wishes


Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson SPaRC
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 
020 7848 6810