[Histonet] pH of Roche Reaction Buffer

Pairan, Kelly Kelly.Pairan at ohiohealth.com
Fri Jul 11 10:19:20 CDT 2025


Good Morning,
How are you pHing the reaction buffer for the Ventana Benchmark Ultra?  Roche doesn't have a published pH for diluted reaction buffer.  CAP requirement ANP.22500 states that pH must be tested when a new batch is prepared or received.  Are you testing the reaction buffer after you dilute the concentrate (ie: preparing a new batch)?

Thanks,
Kelly

Kelly Pairan, HT(ASCP)CM, HQIPCM
Technical Scientist Anatomic Pathology and Cytology
OhioHealth Laboratory Systems
Suite 210 North Medical Building
3535 Olentangy River Rd
Columbus, OH 43214

Email: kelly.pairan at ohiohealth.com<mailto:kelly.pairan at ohiohealth.com>
Work: (614) 566-3575
Cell: (614) 312-0104



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