[Histonet] Histonet Digest, Vol 261, Issue 5

[John O’Brien]

Be sure stainer is not expired 
John IMEB Inc
Fresh stain less then 1 year old is best option we find

> On Aug 20, 2025, at 10:09 AM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
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>   1. Nuclear fast red counterstain (Histology)
>   2. Re: Nuclear fast red counterstain (John Kiernan)
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> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Tue, 19 Aug 2025 17:31:00 +0000
> From: Histology <histo at pathlab.us>
> To: Histonet <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Nuclear fast red counterstain
> Message-ID: <94015281c785445a89549531612cc2fc at pathlab.us>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi all,
> 
> Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.
> 
> Thanks as always Histonet!!
> 
> Mehndi Helgren
> Lab Manager
> 757-664-7901
> Dominion Pathology Labs.
> 733 Boush St. Suite 200
> Norfolk, VA  23510
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Tue, 19 Aug 2025 19:32:29 +0000
> From: John Kiernan <jkiernan at uwo.ca>
> To: Histology <histo at pathlab.us>, Amrit Samra via Histonet
>    <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Nuclear fast red counterstain
> Message-ID:
>    <YT3PR01MB1082091292C9E6C777C08005BA530A at YT3PR01MB10820.CANPRD01.PROD.OUTLOOK.COM>
>    
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Insist on a solution made with nuclear fast red powder that has been certified by the Biological Stain Commission. Then you will know the dye really is CI  60760 and also that it works properly as a stain. See https://pubmed.ncbi.nlm.nih.gov/17510813/
> [https://cdn.ncbi.nlm.nih.gov/pubmed/persistent/pubmed-meta-image-v2.jpg]<https://pubmed.ncbi.nlm.nih.gov/17510813/>
> Certification procedures for nuclear fast red (Kernechtrot), CI 60760<https://pubmed.ncbi.nlm.nih.gov/17510813/>
> Abstract Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain following histochemical procedures that yield blue products. The dye has also been used as a histochemical and colorimetric reagent for calcium. Unsatisfactory samples of nuclear fast red are encountered occasionally, and confusion has ...
> pubmed.ncbi.nlm.nih.gov
> See also  https://biologicalstaincommission.org/new-dyes/, and search down the page for the item on nuclear fast red. This includes a warning about products that are sold as "certified" stains but have not been tested by the BSC.
> 
> Hoping this helps.  John Kiernan.
> = = =
> ________________________________
> From: Histology via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, August 19, 2025 12:31 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Nuclear fast red counterstain
> 
> Hi all,
> 
> Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.
> 
> Thanks as always Histonet!!
> 
> Mehndi Helgren
> Lab Manager
> 757-664-7901
> Dominion Pathology Labs.
> 733 Boush St. Suite 200
> Norfolk, VA  23510
> 
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> End of Histonet Digest, Vol 261, Issue 5
> ****************************************