[Histonet] Nuclear fast red counterstain

[John Kiernan]

Insist on a solution made with nuclear fast red powder that has been certified by the Biological Stain Commission. Then you will know the dye really is CI  60760 and also that it works properly as a stain. See https://pubmed.ncbi.nlm.nih.gov/17510813/
[https://cdn.ncbi.nlm.nih.gov/pubmed/persistent/pubmed-meta-image-v2.jpg]<https://pubmed.ncbi.nlm.nih.gov/17510813/>
Certification procedures for nuclear fast red (Kernechtrot), CI 60760<https://pubmed.ncbi.nlm.nih.gov/17510813/>
Abstract Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain following histochemical procedures that yield blue products. The dye has also been used as a histochemical and colorimetric reagent for calcium. Unsatisfactory samples of nuclear fast red are encountered occasionally, and confusion has ...
pubmed.ncbi.nlm.nih.gov
See also  https://biologicalstaincommission.org/new-dyes/, and search down the page for the item on nuclear fast red. This includes a warning about products that are sold as "certified" stains but have not been tested by the BSC.

Hoping this helps.  John Kiernan.
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________________________________
From: Histology via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, August 19, 2025 12:31 PM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Nuclear fast red counterstain

Hi all,

Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.

Thanks as always Histonet!!

Mehndi Helgren
Lab Manager
757-664-7901
Dominion Pathology Labs.
733 Boush St. Suite 200
Norfolk, VA  23510

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